In Vitro Accumulation of Complexes of Endotoxin and Low-Density Lipoproteins by Macrophages and Arterial Wall

Binding and uptake of complexes of endotoxin and low-density lipoproteins (LPS--LDL) in the arterial wall and mononuclear phagocytes were studied under in vitro conditions. Incubation of aortic explants from Wistar rats with complexes of ¹²⁵I--LDL and S. minnesota R595 LPS or ¹²⁵I--LDL was accompani...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2009-02, Vol.147 (2), p.189-192
Main Authors: Schwartz, Y. Sh, Dushkin, M. I
Format: Article
Language:English
Subjects:
Animals
Aorta
Aorta - metabolism
atherosclerosis
bacterial lipopolysaccharides
Biological Transport - physiology
Biomedical and Life Sciences
Biomedicine
Biophysics and Biochemistry
Cell Biology
Cells, Cultured
Endotoxins
Explants
In Vitro Techniques
Internal Medicine
Laboratory Medicine
Leukocytes (mononuclear)
Lipopolysaccharides
Lipopolysaccharides - metabolism
Lipoproteins
Lipoproteins (low density)
Lipoproteins, LDL - metabolism
low-density lipoproteins
LPS > LDL complexes
Macrophages
Macrophages, Peritoneal - metabolism
Pathology
Peritoneum
Phagocytes
Protein Binding
Rats
Rats, Wistar
Vascular system
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Summary:Binding and uptake of complexes of endotoxin and low-density lipoproteins (LPS--LDL) in the arterial wall and mononuclear phagocytes were studied under in vitro conditions. Incubation of aortic explants from Wistar rats with complexes of ¹²⁵I--LDL and S. minnesota R595 LPS or ¹²⁵I--LDL was accompanied by a 6-fold increase in binding (0°C) and 2-fold increase in the uptake (37°C) of LDL--LPS complexes as compared to free LDL. Binding and degradation of ¹²⁵I--LDL--LPS complexes in the culture of peritoneal macrophages were higher compared to the corresponding parameters for free ¹²⁵I--LDL. Our results suggest that the formation of LDL--LPS complexes is followed by the increased binding and accumulation of LDL in the arterial wall and macrophages. These changes probably induce the cascade of major atherogenic events in the vascular wall.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-009-0488-0