Comparison of ion channel distribution and expression in cardiomyocytes of canine pulmonary veins versus left atrium

Cardiomyocytes in pulmonary vein (PV) sleeves are important in atrial fibrillation (AF), but underlying mechanisms are poorly understood. Pulmonary veins have different ionic current properties compared to left atrium, with pulmonary vein inward-rectifier currents being smaller and delayed-rectifier...

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Bibliographic Details
Published in:Cardiovascular research 2005, Vol.65 (1), p.104-116
Main Authors: MELNYK, Peter, EHRLICH, Joachim R, POURRIER, Marc, VILLENEUVE, Louis, CHA, Tae-Joon, NATTEL, Stanley
Format: Article
Language:English
Subjects:
Animals
Arrhythmias, Cardiac - metabolism
Biological and medical sciences
Blotting, Western - methods
Calcium Channels, L-Type - analysis
Cardiac dysrhythmias
Cardiology. Vascular system
CHO Cells
Cricetinae
Dogs
Electrophysiology
ERG1 Potassium Channel
Ether-A-Go-Go Potassium Channels
Female
Heart
Heart Atria - cytology
Heart Atria - metabolism
Immunohistochemistry - methods
Ion Channels - analysis
Ion Channels - metabolism
KCNQ Potassium Channels
KCNQ1 Potassium Channel
Male
Medical sciences
Microscopy, Confocal
Myocytes, Cardiac - metabolism
Patch-Clamp Techniques
Potassium Channels - analysis
Potassium Channels, Inwardly Rectifying - analysis
Potassium Channels, Voltage-Gated - analysis
Pulmonary Veins - cytology
Pulmonary Veins - metabolism
Sodium-Calcium Exchanger - analysis
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Summary:Cardiomyocytes in pulmonary vein (PV) sleeves are important in atrial fibrillation (AF), but underlying mechanisms are poorly understood. Pulmonary veins have different ionic current properties compared to left atrium, with pulmonary vein inward-rectifier currents being smaller and delayed-rectifier currents larger than in left atrium. Expression and distribution of the inward-rectifier subunits Kir2.1 and Kir2.3, the rapid delayed-rectifier alpha-subunit ERG, the slow delayed-rectifier alpha-subunit KvLQT1, the beta-subunit minK, the L-type Ca(2+)-subunit Ca(v)1.2, and the Na(+),Ca(2+)-exchanger were quantified by Western blot on isolated cardiomyocytes and localized by immunohistochemistry in tissue sections obtained from canine hearts. Western blotting indicated significantly greater expression of ERG (by 28%, P
ISSN:0008-6363
1755-3245
DOI:10.1016/j.cardiores.2004.08.014