Opsonic function of bronchoalveolar lavage IgG after pneumococcal vaccination in HIV-infected and uninfected adults

To the Editor: Pneumococcal conjugate vaccination is immunogenic both systemically and at the mucosal surface,1 reducing carriage and disease, thus resulting in herd immunity.2 Pneumococcal conjugate vaccination is effective at preventing disease in HIV-infected adults,3 despite impairments in antip...

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Bibliographic Details
Published in:	Journal of allergy and clinical immunology 2009-06, Vol.123 (6), p.1420-1421
Main Authors:	Tam, Jerry C.H, Grant, Nicola L., MSc, Freire-Moran, Laura, MSc, Tolmie, Helen, PhD, French, Neil, FRCP, PhD, Gordon, Stephen B., FRCP, MD
Format:	Article
Language:	eng
Subjects:	Adult AIDS-Related Opportunistic Infections - prevention & control Allergy and Immunology Bacteria Bronchoalveolar Lavage Fluid - immunology Bronchoalveolar Lavage Fluid - microbiology Female HIV Infections - complications HIV Infections - immunology Human immunodeficiency virus Humans Immunoglobulin G - immunology Male Medical research Middle Aged Opsonin Proteins - immunology Pneumococcal Infections - prevention & control Pneumococcal Vaccines - administration & dosage Pneumococcal Vaccines - immunology Streptococcus pneumoniae Vaccination Vaccines Vaccines, Conjugate - administration & dosage Vaccines, Conjugate - immunology
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Summary:	To the Editor: Pneumococcal conjugate vaccination is immunogenic both systemically and at the mucosal surface,1 reducing carriage and disease, thus resulting in herd immunity.2 Pneumococcal conjugate vaccination is effective at preventing disease in HIV-infected adults,3 despite impairments in antipneumococcal opsonic function in both serum4 and bronchoalveolar lavage (BAL).5 We wished to determine whether improvement in the opsonic function of IgG in BAL could be detected after vaccination. BAL was centrifuged, the supernatant was stored at -80°C, and the concentrations of IgG against each of the pneumococcal serotypes were confirmed by ELISA.1 The opsonic function of BAL IgG was assessed as previously described.5 Briefly, BAL IgG was concentrated 10-fold by using IgG affinity chromatography columns (Pierce Biotechnology, Rockford, Ill).
ISSN:	0091-6749 1097-6825