Systematic identification and molecular characterization of genes differentially expressed in breast and ovarian cancer

The identification of novel disease‐associated genes in gynaecological tumours has important implications for understanding the process of tumourigenesis and the development of novel treatment regimens. cDNA libraries from disease tissues may represent a valuable source to identify such genes. Recen...

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Bibliographic Details
Published in:The Journal of pathology 2005-01, Vol.205 (1), p.21-28
Main Authors: Dahl, Edgar, Sadr-Nabavi, Ariane, Klopocki, Eva, Betz, Beate, Grube, Susanne, Kreutzfeld, Rene, Himmelfarb, Marina, An, Han-Xiang, Gelling, Stephen, Klaman, Irina, Hinzmann, Bernd, Kristiansen, Glen, Grützmann, Robert, Kuner, Ruprecht, Petschke, Beate, Rhiem, Kerstin, Wiechen, Kai, Sers, Christine, Wiestler, Otmar, Schneider, Achim, Höfler, Heinz, Nährig, Jörg, Dietel, Manfred, Schäfer, Reinhold, Rosenthal, André, Schmutzler, Rita, Dürst, Matthias, Meindl, Alfons, Niederacher, Dieter
Format: Article
Language:English
Subjects:
Biological and medical sciences
breast cancer
Breast Neoplasms - genetics
Carcinoma, Ductal, Breast - genetics
differentially expressed gene
DNA, Complementary - genetics
DNA, Neoplasm - genetics
electronic Northern
Expressed Sequence Tags
Female
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genetic Markers
Gynecology. Andrology. Obstetrics
Humans
Investigative techniques, diagnostic techniques (general aspects)
Mammary gland diseases
Medical sciences
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
ovarian cancer
Ovarian Neoplasms - genetics
Ovarian Neoplasms - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Reverse Transcriptase Polymerase Chain Reaction - methods
Tumors
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Summary:The identification of novel disease‐associated genes in gynaecological tumours has important implications for understanding the process of tumourigenesis and the development of novel treatment regimens. cDNA libraries from disease tissues may represent a valuable source to identify such genes. Recently, a bio‐informatic procedure based on an ‘electronic Northern’ approach was established to screen expressed sequence tag (EST) libraries for genes differentially expressed in tumour and normal tissues, and identified 450 candidate genes differentially expressed in breast and ovarian cancer. In this report, the validation of an initial set of 40 candidate genes, which were selected due to their localization in chromosomal regions frequently altered in gynaecological tumours, is described. Differential expression of 29 of these genes, including three uncharacterized novel genes, was confirmed by applying cancer profiling arrays with 106 matched pairs of tumour/normal cDNAs and quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) on 60 clinical specimens. The majority of these differentially expressed genes have not been described previously in the context of breast and ovarian cancer, and may constitute novel diagnostic markers for these tumour entities. Copyright © 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
ISSN:0022-3417
1096-9896
DOI:10.1002/path.1687