Development of a Rapid Resolution Liquid Chromatographic Method for Simultaneous Analysis of Four Alkaloids in Rhizoma coptidis Under Different Cultivation Conditions

A sensitive and specific method using rapid resolution liquid chromatography coupled with UV-Vis detection was developed for fingerprint analysis of Rhizoma coptidis and simultaneous determination of 4 alkaloids: jatrorrhizine, coptisine, palmatine, and berberine. Samples of R. coptidis grown under...

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Bibliographic Details
Published in:Journal of AOAC International 2009-03, Vol.92 (2), p.663-671
Main Authors: Qiao, Yan-Ling, Sheng, Yu-Xin, Wang, Li-Qun, Zhang, Jin-Lan
Format: Article
Language:English
Subjects:
Alkaloids
antinutritional factors
berberastine
berberine
Berberine - analogs & derivatives
Berberine - analysis
Berberine Alkaloids - analysis
Berberine Alkaloids - standards
chemical constituents of plants
Chemical properties
chemical structure
Chromatography, Liquid - methods
Chromatography, Liquid - standards
Chromatography, Liquid - statistics & numerical data
columbramine
Coptis
Coptis - chemistry
Coptis - growth & development
Coptis japonica
coptisine
cultivars
dietary supplements
epi-berberine
herbal medicines
herbs
Identification and classification
jatrorrhizine
Liquid chromatography
mass spectrometry
Measurement
Medicinal plants
Methods
Oriental traditional medicine
palmatine
perennials
plant extracts
quality control
quantitative analysis
Ranunculaceae
rapid methods
Reference Standards
reversed-phase high performance liquid chromatography
Rhizoma coptidis
Rhizome - chemistry
rhizomes
Sensitivity and Specificity
Stems (Botany)
ultraviolet-visible spectroscopy
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Summary:A sensitive and specific method using rapid resolution liquid chromatography coupled with UV-Vis detection was developed for fingerprint analysis of Rhizoma coptidis and simultaneous determination of 4 alkaloids: jatrorrhizine, coptisine, palmatine, and berberine. Samples of R. coptidis grown under different cultivation conditions and from different habitats were analyzed. The analysis was performed using a reversed-phase octylsilyl (C8) column and gradient elution. The mobile phase consisted of acetonitrile and 20 mmol/L KH2PO4. Each analysis was completed within 3.5 min. The method showed good linearity within test ranges of 4.75-47.50 μg/mL for jatrorrhizine, 20.60-164.80 μg/mL for coptisine, 18.07-180.73 μg/mL for palmatine, and 89.70-717.57 μg/mL for berberine. The method showed good precision, repeatability, and stability for quantification of the 4 alkaloids. The lower limit of detection was 0.19 ng for jatrorrhizine, 0.21 ng for coptisine, 0.15 ng for palmatine, and 0.14 ng for berberine. The lower limit of quantification was 0.57 ng for jatrorrhizine, 0.82 ng for coptisine, 0.55 ng for palmatine, and 0.27 ng for berberine. The overall recovery ranged from 96.30 to 104.10% for the 4 alkaloids. The method is accurate, rapid, and convenient, and it is suitable for routine quality control of R. coptidis.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/92.2.663