Application of HILIC stationary phase to determination of dimethindene maleate in topical gel

A novel high performance liquid chromatography method for the determination of dimethindene maleate in pharmaceutical gel using hydrophilic interaction liquid chromatography (HILIC) with UV detection was developed and validated. Following optimal conditions for the analysis of dimethindene maleate w...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2009-08, Vol.50 (1), p.23-26
Main Authors: Matysová, L., Havlíková, L., Hájková, R., Krivda, A., Solich, P.
Format: Article
Language:English
Subjects:
Administration, Topical
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Dimethindene
Dimethindene - administration & dosage
Dimethindene - analysis
Fundamental and applied biological sciences. Psychology
Gels
General pharmacology
HILIC
Histamine H1 Antagonists - administration & dosage
Histamine H1 Antagonists - analysis
HPLC
Medical sciences
Pharmaceuticals
Pharmacology. Drug treatments
Reference Standards
Spectrophotometry, Ultraviolet
UV detection
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Summary:A novel high performance liquid chromatography method for the determination of dimethindene maleate in pharmaceutical gel using hydrophilic interaction liquid chromatography (HILIC) with UV detection was developed and validated. Following optimal conditions for the analysis of dimethindene maleate were used: analytical column SeQuant ZIC ®–HILIC (50 mm × 2.1 mm, 5 μm), and mobile phase consisted of a mixture of acetonitrile and aqueous solution of acetic acid (25 mM) and ammonium acetate (2.5 mM) (87.5:12.5, v:v). The analysis time was less than 3 min at a flow rate of 0.3 ml min −1. UV detection was performed at 258 nm. The method was validated and system suitability parameters were evaluated. The method is suitable for application for routine determination of dimethindene maleate in topical pharmaceutical preparation.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2009.03.032