Glutamine is a key regulator for amino acid-controlled cell growth through the mTOR signaling pathway in rat intestinal epithelial cells

Amino acids, especially branched-chain amino acids such as l-leucine, have been shown to regulate activation of p70 S6 kinase and phosphorylation of 4E-BP1 through the mTOR signaling pathway. In our recent study, l-arginine was also shown to activate the mTOR signaling pathway in rat intestinal epit...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2005-01, Vol.326 (1), p.174-180
Main Authors: Nakajo, Tohru, Yamatsuji, Tomoki, Ban, Hidetoshi, Shigemitsu, Kaori, Haisa, Minoru, Motoki, Takayuki, Noma, Kazuhiro, Nobuhisa, Tetsuji, Matsuoka, Junji, Gunduz, Mehmet, Yonezawa, Kazuyoshi, Tanaka, Noriaki, Naomoto, Yoshio
Format: Article
Language:English
Subjects:
4E-BP1
Amino Acids - pharmacology
Animals
Arginine - pharmacology
Cell Line
Cell Proliferation - drug effects
Dose-Response Relationship, Drug
Gene Expression Regulation - drug effects
Glutamine - pharmacology
IEC16
IEC18
Intestinal Mucosa - drug effects
Intestinal Mucosa - physiology
l-Arginine
l-Glutamine
l-Leucine
Leucine - pharmacology
mTOR
p70 S6 kinase
Protein Kinases - metabolism
Rats
Ribosomal Protein S6 Kinases, 70-kDa - metabolism
Signal Transduction - drug effects
Signal Transduction - physiology
TOR Serine-Threonine Kinases
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Summary:Amino acids, especially branched-chain amino acids such as l-leucine, have been shown to regulate activation of p70 S6 kinase and phosphorylation of 4E-BP1 through the mTOR signaling pathway. In our recent study, l-arginine was also shown to activate the mTOR signaling pathway in rat intestinal epithelial cells. l-Glutamine is an amino acid that is required for culturing of numerous cell types, including rat intestinal epithelial cells. In this study, we showed that l-glutamine inhibited the activation of p70 S6 kinase and phosphorylation of 4E-BP1 induced by arginine or leucine in rat intestinal epithelial cells. Although the molecular mechanism of l-glutamine-induced inhibition of the mTOR signaling pathway is still unknown, the presence of this novel signal pathway may indicate that individual amino acids play specific roles for cellular proliferation and growth.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.11.015