A strategy to verify the absence of the pgm locus in Yersinia pestis strain candidates for select agent exemption

Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm − strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains...

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Bibliographic Details
Published in:Journal of microbiological methods 2009-06, Vol.77 (3), p.316-319
Main Authors: Jenkins, Amy L., Worsham, Patricia L., Welkos, Susan L.
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Humans
Microbiology
Mutation
Pgm locus
Pigments, Biological - genetics
Pigments, Biological - metabolism
Plague - microbiology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Select agent rules
United States
United States Dept. of Health and Human Services - legislation & jurisprudence
Virulence Factors - genetics
Virulence Factors - metabolism
Yersinia pestis
Yersinia pestis - genetics
Yersinia pestis - metabolism
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Summary:Yersinia pestis is a Department of Health and Human Services select agent as defined in federal regulations. Certain attenuated strains of Y. pestis, such as the pgm − strain, are exempt from these regulations. Herein we describe a strategy to verify the absence of the pgm locus in Y. pestis strains being considered as candidates for select agent exemption by PCR analysis of virulence-associated genes.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2009.02.013