Nucleotide sequence of the matrix protein gene of avian paramyxovirus, serotype 3b: evidence on another member of the suggested new genus of the subfamily Paramyxovirinae

The complete nucleotide sequence of the gene encoding the matrix protein (M) of the avian paramyxovirus, serotype 3b (APMV-3b), has been determined by means of the direct sequencing of viral RNA using reverse transcriptase reaction. The adjacent portions of the neighboring phosphoprotein (P) and fus...

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Bibliographic Details
Published in:Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2005, Vol.28 (1), p.37-51
Main Authors: Shihmanter, Esther, Panshin, A., Lipkind, M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Avian paramyxovirus
Avulavirus
Avulavirus - classification
Avulavirus - genetics
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Matrix protein gene
Microbiology
Miscellaneous
Molecular Sequence Data
Newcastle disease virus
Paramyxovirus
Paramyxovirus aviaire
Phylogeny
Protein de la matrice
Rubulavirus
Sequenc [formula omitted]age
Sequence Alignment
Sequence Homology, Amino Acid
Sequencing
Species Specificity
Viral Matrix Proteins - chemistry
Viral Matrix Proteins - genetics
Virology
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Summary:The complete nucleotide sequence of the gene encoding the matrix protein (M) of the avian paramyxovirus, serotype 3b (APMV-3b), has been determined by means of the direct sequencing of viral RNA using reverse transcriptase reaction. The adjacent portions of the neighboring phosphoprotein (P) and fusion (F) protein genes were also sequenced that permitted to determine the consensus sequence of the viral genome, the poly(A) tract, downstream and upstream non-coding portions of the P and F genes, respectively, as well as the corresponding intergenic regions. The gene is 1478 nucleotides long with a protein-coding sequence of 1194 nucleotides. The deduced protein consists of 398 amino acids with a calculated MW 44,465. According to the multalignment and phylogenetic analyses, the APMV-3b M protein has shown the closest relatedness towards Newcastle disease virus (NDV) which has recently been suggested to be excluded from the Rubulavirus genus and assigned (together with APMV-6) to a new Avulavirus genus within the subfamily Paramyxovirinae of the Paramyxoviridae family. On the basis of the M protein genetic multalignment, phylogenetic relationships, bipartite nuclear localization signal identification in combination with the cysteine residues distribution, and by the degree of intrageneric heterogeneity, the APMV-3b is proposed to be another member (together with NDV and APMV-6) of the new genus. La séquence nucléotidique complète du gène codant pour la protéine de la matrice d'un paramyxovirus aviaire, sérotype 3b (APMV-3b) a été déterminée par séquençage direct de l'ARN viral a l'aide d'une réaction ‘reverse transcriptase’. Les régions adjacentes des gènes de la phosphoprotéine (P) et de la protéine de fusion (F) ont aussi été séquençée afin de déterminer la séquence consensuelle du génome viral, de la zone poly(A), des régions non codantes en amont et en aval des gènes P et F respectivement, ainsi que les régions correspondantes entre ces différents gènes. Le gène est constitué de 1478 nucléotides dont une région codante de 1194 nucléotides. La protéine déduite est formée de 398 amino acides et à un poids moléculaire estimé de 44,465. Selon l'alignement multiple et l'analyse de l'arbre phylogénétique, la protéine M de l'APMV-3b montre la plus grande proximité avec le virus de la maladie de Newcastle (NDV) qui a été récemment proposé pour être transféré du genre Rubulavirus au nouveau genre Avulavirus (avec APMV-6) au sein de la sous-famille Paramyxovirinae
ISSN:0147-9571
1878-1667
DOI:10.1016/j.cimid.2004.03.010