Characterization of a novel rat gene RTAP2a, screened by cross-reactive SEREX, restrictedly expressed in testis

Genes involved in tumorigenesis may be targets for cancer immunotherapy. For many tumor types, molecular signatures do not yet exist. Methods that identify novel, tissue-specific tumor-related genes therefore have potentially important implications for developing cancer immunotherapy. Here, we used...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2009-06, Vol.107 (6), p.589-595
Main Authors: Yang, Hanshuo, Wang, Chunting, Wang, Rui, Deng, Hongxin, Ding, Zhenyu, Yang, Jinliang, Lu, You, Li, Jiong, Zhang, Peng, Mao, Yongqiu, Kan, Bing, Wei, Lin, Peng, Feng, Wei, Yuquan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antigens, Neoplasm - physiology
Base Sequence
Biological and medical sciences
Biotechnology
Cell Line, Tumor
Cross-reactive SEREX
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
GENETIC TRANSFORMATION
Humans
IMMUNOTHERAPIE
IMMUNOTHERAPY
INMUNOTERAPIA
Male
Molecular Sequence Data
Neoplasm Proteins - physiology
NEOPLASMAS
NEOPLASME
NEOPLASMS
PCR
Protein Isoforms
RAT
RATA
RATS
RTAP2
SEREX
Splice variant
TESTES
Testicular Neoplasms - immunology
TESTICULE
TESTICULOS
Testis
Testis - immunology
TRANSCRIPCION
TRANSCRIPTION
TRANSFORMACION GENETICA
TRANSFORMATION GENETIQUE
VARIANT
VARIANTES
VARIANTS
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Summary:Genes involved in tumorigenesis may be targets for cancer immunotherapy. For many tumor types, molecular signatures do not yet exist. Methods that identify novel, tissue-specific tumor-related genes therefore have potentially important implications for developing cancer immunotherapy. Here, we used cross-reactive serological analysis of recombinant cDNA expression (CR-SEREX) to identify the novel testicular cancer-related gene, rat testicular antigenic protein 2 (RTAP2). We identified two RTAP2 splice variants (RTAP2a and RTAP2b) encoding 191 and 358 amino acid proteins, respectively. RTAP2a and RTAP2b proteins have identical N-termini but different C-terminal regions arising from alternative splicing of exon 4. Bioinformatics and reverse transcription-polymerase chain reaction (RT-PCR) revealed that the RTAP2a transcript was expressed selectively in adult testis and several tumor cell lines, while RTAP2b was ubiquitous. Our results suggest that RTAP2a may contribute to testicular development and tumorigenesis, and may be a candidate gene for cancer immunotherapy.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2009.02.005