Recombination of two porcine circovirus type 2 strains

Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2009-05, Vol.154 (5), p.875-879
Main Authors: Lefebvre, David J, Van Doorsselaere, J, Delputte, P. L, Nauwynck, H. J
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Biomedical and Life Sciences
Biomedicine
Brief Report
Cell culture
Cells, Cultured
Circoviridae Infections - veterinary
Circoviridae Infections - virology
Circovirus - classification
Circovirus - genetics
Cloning
DNA, Viral - genetics
Fundamental and applied biological sciences. Psychology
Genome, Viral
Genomes
Hogs
Infectious Diseases
Medical Microbiology
Microbiology
Miscellaneous
Molecular Sequence Data
Phylogenetics
Porcine circovirus
Proteins
Reassortant Viruses - classification
Reassortant Viruses - genetics
Recombination, Genetic
Sequence Alignment
Sequence Analysis, DNA
Swine - virology
Swine Diseases - virology
Virology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Pigs can be concurrently infected with different PCV2 strains. In this study, a cell-culture-adapted PCV2 strain, originating from a PMWS-affected pig, was purified by limiting dilution. Three different strains were obtained, and one of them was a perfect mosaic of the other two, with recombination breakpoints in ORF1 and ORF2. Incongruence was observed between phylogenetic trees constructed with the whole genome, ORF1 and ORF2. Amplification of ORF1 and ORF2 from original material, followed by cloning and sequencing, resulted in sequences corresponding to the parental strains, but not with the mosaic strain. These results demonstrate that PCV2 can undergo recombination.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-009-0379-8