Rolling-circle amplification of viral DNA genomes using phi29 polymerase

Techniques for the single-step amplification of whole genomes have been developed into powerful tools for phylogenetic analyses, epidemiological studies and studies on genome organization. Recently, the bacteriophage phi29 DNA polymerase has been used for the efficient amplification of circular DNA...

Full description

Saved in:
Bibliographic Details
Published in:Trends in microbiology (Regular ed.) 2009-05, Vol.17 (5), p.205-211
Main Authors: Johne, Reimar, Müller, Hermann, Rector, Annabel, van Ranst, Marc, Stevens, Hans
Format: Article
Language:English
Subjects:
Animals
Bacteriophages - enzymology
DNA Replication
DNA Viruses - genetics
DNA Viruses - isolation & purification
DNA, Circular - metabolism
DNA, Viral - metabolism
DNA-Directed DNA Polymerase - metabolism
Humans
Internal Medicine
Models, Biological
Plants - virology
Viral Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Techniques for the single-step amplification of whole genomes have been developed into powerful tools for phylogenetic analyses, epidemiological studies and studies on genome organization. Recently, the bacteriophage phi29 DNA polymerase has been used for the efficient amplification of circular DNA viral genomes without the need of specific primers by a rolling-circle amplification (RCA) mechanism. Various protocols have been applied for detection of novel viruses, for differentiation between circular and linear forms of viral genomes and for generation of infectious genomic clones directly from specimens. Here, we summarize the broad application of the RCA technique to DNA viruses infecting humans, animals and plants.
ISSN:0966-842X
1878-4380
DOI:10.1016/j.tim.2009.02.004