The in vitro kinetics of senescence of Fischer 344 rat embryo fibroblasts

The kinetics of replicative senescence have been determined in three independent cultures of fibroblasts derived from Fischer rat embryos (FREF). In each case the growth fraction was measured by immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and by metabolic labeling using...

Full description

Saved in:
Bibliographic Details
Published in:Biogerontology (Dordrecht) 2009-06, Vol.10 (3), p.285-289
Main Authors: Faragher, Richard G. A., Kill, Ian R.
Format: Article
Language:English
Subjects:
Aging
Animals
Antigens
Biological and medical sciences
Biomedical and Life Sciences
Bromodeoxyuridine - metabolism
Cell Biology
Cell cycle
Cell death
Cell Proliferation
Cells, Cultured
Cellular Senescence
Development. Metamorphosis. Moult. Ageing
Developmental Biology
Embryo, Mammalian - cytology
Embryos
Fibroblasts - metabolism
Fibroblasts - physiology
Fundamental and applied biological sciences. Psychology
Geriatrics/Gerontology
Kinetics
Laboratories
Life Sciences
Metabolism
Proliferating Cell Nuclear Antigen - metabolism
Rats
Rats, Inbred F344
Research Article
Senescence
Vertebrates: anatomy and physiology, studies on body, several organs or systems
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The kinetics of replicative senescence have been determined in three independent cultures of fibroblasts derived from Fischer rat embryos (FREF). In each case the growth fraction was measured by immunocytochemical staining for proliferating cell nuclear antigen (PCNA) and by metabolic labeling using bromodeoxyuridine (BrdU). FREF cultures entered senescence at an average of 20.4 population doublings. The growth fraction declined smoothly as measured by both kinetic techniques. The average rate of decline of the growth fraction observed using BrdU was −0.79 ± 0.12% population doubling −1 which was closely comparable to the loss of PCNA reactivity (−0.80 ± 0.11% population doubling −1 ). We conclude that senescence in FREF cultures occurs as the result of a progressive loss of the culture growth fraction rather than a sudden abrupt collapse.
ISSN:1389-5729
1573-6768
DOI:10.1007/s10522-008-9198-7