The Gs-Linked receptor GPR3 maintains meiotic arrest in mammalian oocytes

Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2004-12, Vol.306 (5703), p.1947-1950
Main Authors: MEHLMANN, Lisa M, SAEKI, Yoshinaga, TANAKA, Shigeru, BRENNAN, Thomas J, EVSIKOV, Alexei V, PENDOLA, Frank L, KNOWLES, Barbara B, EPPIG, John J, JAFFE, Laurinda A
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Animals
Biological and medical sciences
Chondroitin Sulfate Proteoglycans - genetics
Chondroitin Sulfate Proteoglycans - metabolism
Expressed Sequence Tags
Female
Fundamental and applied biological sciences. Psychology
Granulosa Cells - physiology
Heterotrimeric GTP-Binding Proteins - metabolism
In Situ Hybridization
Lectins, C-Type
Ligands
Luteinizing Hormone - metabolism
Mammalian female genital system
Meiosis
Metaphase
Mice
Mice, Inbred C57BL
Mice, Knockout
Mitosis
Morphology. Physiology
Oocytes - physiology
Ovarian Follicle - physiology
Receptors, G-Protein-Coupled - genetics
Receptors, G-Protein-Coupled - physiology
RNA - genetics
RNA - metabolism
Versicans
Vertebrates: reproduction
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Summary:Mammalian oocytes are held in prophase arrest by an unknown signal from the surrounding somatic cells. Here we show that the orphan Gs-linked receptor GPR3, which is localized in the oocyte, maintains this arrest. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype can be reversed by injection of Gpr3 RNA into the oocytes. Thus, the GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle and is crucial for the regulation of meiosis.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1103974