Plasmodium falciparum: sequence diversity and antibody recognition of the Merozoite surface protein-2 (MSP-2) in Brazilian Amazonia

The merozoite surface protein-2 (MSP-2) of Plasmodium falciparum comprises repeats flanked by dimorphic domains defining the allelic families FC27 and IC1. Here, we examined sequence diversity at the msp-2 locus in Brazil and its impact on MSP-2 antibody recognition by local patients. Only 25 unique...

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Bibliographic Details
Published in:Experimental parasitology 2004-11, Vol.108 (3), p.114-125
Main Authors: Tonon, Angela Pedroso, Hoffmann, Erika Hellena Esther, Silveira, Lucimeire Antonelli da, Ribeiro, Adálton Guimarães, Gonçalves, Clara Rosa da Silva, Ribolla, Paulo Eduardo Martins, Wunderlich, Gerhard, Ferreira, Marcelo Urbano
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Alleles
Amino Acid Sequence
Animals
Antibodies, Protozoan - blood
Antibodies, Protozoan - immunology
Antigenic diversity
Antigens, Protozoan - chemistry
Antigens, Protozoan - immunology
Base Sequence
Brazil - epidemiology
Child
Child, Preschool
Cross Reactions
degrees of freedom
ELISA
Enzyme-Linked Immunosorbent Assay
Female
Genetic Variation
glutathione- S-transferase
GST
Humans
Immunoglobulin G - immunology
Immunoglobulin M - immunology
Infant
Malaria parasites
Malaria, Falciparum - epidemiology
Malaria, Falciparum - parasitology
Male
merozoite surface protein-1
merozoite surface protein-2
Middle Aged
Molecular Sequence Data
MSP-1
MSP-2
PCR
Plasmodium falciparum
Plasmodium falciparum - genetics
Plasmodium falciparum - immunology
polymerase chain reaction
Protozoa
Protozoan Proteins - chemistry
Protozoan Proteins - immunology
SDS–PAGE
single nucleotide polymorphism
SNP
sodium dodecyl sulfate–polyacrylamide gel electrophoresis
standard deviation
Vaccine
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Summary:The merozoite surface protein-2 (MSP-2) of Plasmodium falciparum comprises repeats flanked by dimorphic domains defining the allelic families FC27 and IC1. Here, we examined sequence diversity at the msp-2 locus in Brazil and its impact on MSP-2 antibody recognition by local patients. Only 25 unique partial sequences of msp-2 were found in 61 isolates examined. The finding of identical msp-2 sequences in unrelated parasites, collected 6–13 years apart, suggests that no major directional selection is exerted by variant-specific immunity in this malaria-endemic area. To examine antibody cross-reactivity, recombinant polypeptides derived from locally prevalent and foreign MSP-2 variants were used in ELISA. Foreign IC1-type variants, such as 3D7 (currently tested for human vaccination), were less frequently recognized than FC27-type and local IC1-type variants. Antibodies discriminated between local and foreign IC1-type variants, but cross-recognized structurally different local IC1-type variants. The use of evolutionary models of MSP-2 is suggested to design vaccines that minimize differences between local parasites and vaccine antigens.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2004.08.001