Novel potentiometry immunoassay with amplified sensitivity for diphtheria antigen based on Nafion, colloidal Ag and polyvinyl butyral as matrixes

A novel potentiometry immunoassay with amplified sensitivity has been developed for the detection of diphtheria antigen (Diph) via immobilizing diphtheria antibody (anti-Diph) on a platinum electrode based on Nafion, colloidal Ag (Ag), and polyvinyl butyral (PVB) as matrixes in this study. The modif...

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Bibliographic Details
Published in:Journal of biochemical and biophysical methods 2004-11, Vol.61 (3), p.299-311
Main Authors: Tang, Dianping, Yuan, Ruo, Chai, Yaqin, Zhang, Linyan, Zhong, Xia, Dai, Jianyuan, Liu, Yan
Format: Article
Language:English
Subjects:
Antigens - analysis
Antigens - immunology
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Colloidal Ag
Diphtheria
Diphtheria - immunology
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Fluorocarbon Polymers - chemistry
Humans
Immunoassay - instrumentation
Immunoassay - methods
Nafion
Platinum electrode
Polyvinyl butyral
Polyvinyls - chemistry
Potentiometric immunosensor
Reproducibility of Results
Sensitivity and Specificity
Silver - chemistry
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Summary:A novel potentiometry immunoassay with amplified sensitivity has been developed for the detection of diphtheria antigen (Diph) via immobilizing diphtheria antibody (anti-Diph) on a platinum electrode based on Nafion, colloidal Ag (Ag), and polyvinyl butyral (PVB) as matrixes in this study. The modified procedure was further characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The influence and factors influencing the performance of resulting immunosensor were studied in detail. The resulting immunosensor exhibited sigmoid curve with log Diph concentrations, high sensitivity (51.4 mV/decade), wide linear range from 8 to 800 ng ml −1 with a detection limit of 1.5 ng ml −1, rapid potentiometric response (6 months). Analytical results of clinical samples show that the developed immunoassay is comparable with the enzyme-linked immunosorbent assays (ELISAs) method, implying a promising alternative approach for detecting diphtheria antigen in the clinical diagnosis.
ISSN:0165-022X
1872-857X
DOI:10.1016/j.jbbm.2004.06.001