Neudesin, an extracellular heme-binding protein, suppresses adipogenesis in 3T3-L1 cells via the MAPK cascade

Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2009-03, Vol.381 (1), p.75-80
Main Authors: Kimura, Ikuo, Konishi, Morichika, Asaki, Toshiyuki, Furukawa, Naoko, Ukai, Kiyotaka, Mori, Masahiro, Hirasawa, Akira, Tsujimoto, Gozoh, Ohta, Mitsuhiro, Itoh, Nobuyuki, Fujimoto, Masafumi
Format: Article
Language:English
Subjects:
3T3-L1
3T3-L1 Cells
Adipocytes
Adipocytes - enzymology
Adipocytes - physiology
Adipogenesis
Animals
Cell Proliferation
Gene Knockdown Techniques
Heme
Heme-binding protein
MAP Kinase Signaling System
MAPK
Mice
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - physiology
Neudesin
Obesity
RNA Interference
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Summary:Adult mice abundantly express neudesin, an extracellular heme-binding protein with neurotrophic activity, in white adipose tissues. At the early stage of adipocyte differentiation during adipogenesis, however, the expression of neudesin decreased transiently. Neudesin-hemin significantly suppressed adipogenesis in 3T3-L1 cells. The knockdown of neudesin by RNA interference markedly promoted adipogenesis in 3T3-L1 cells and decreased MAPK activation during adipocyte differentiation. The addition or knockdown of neudesin affected the expression of C/EBPα and PPARγ but not of C/EBPβ. These findings suggest that neudesin plays a critical role in the early stage of adipocyte differentiation in which C/EBPβ induces PPARγ and C/EBPα expressions, by controlling the MAPK pathway.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.02.011