Separation of phenolic glycolipids in mycobacterium bovis BCG by reversed-phase high performance liquid chromatography

A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275 nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Myco...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2009-04, Vol.22 (2), p.145-149
Main Authors: Salimi, Mona, Pourabdi, Ladan, Zakeri, Maryam, Abdipour, Nazanin
Format: Article
Language:English
Subjects:
Acetonitriles - chemistry
Chemical Fractionation
Chromatography, High Pressure Liquid - methods
Glycolipids - isolation & purification
Mycobacterium bovis - chemistry
Phenols - isolation & purification
Reproducibility of Results
Solvents - chemistry
Spectrophotometry, Ultraviolet
Water - chemistry
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Summary:A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275 nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0-->80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract.
ISSN:1011-601X