Detection of Novel Organisms Associated with Salpingitis, by Use of 16S rDNA Polymerase Chain Reaction

Although Chlamydia trachomatis and Neisseria gonorrhoeae are established causes of salpingitis, the majority of cases have no known etiology. We used broad-range 16S rDNA polymerase chain reaction to identify novel, possibly uncultivable, bacteria associated with salpingitis and identified bacterial...

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Bibliographic Details
Published in:The Journal of infectious diseases 2004-12, Vol.190 (12), p.2109-2120
Main Authors: Hebb, Jennifer K., Cohen, Craig R., Astete, Sabina G., Bukusi, Elizabeth A., Totten, Patricia A.
Format: Article
Language:English
Subjects:
Adult
Atopobium vaginae
Bacteria
Bacterial diseases
Bacterial diseases of the genital system
Bacterial vaginosis
Base Sequence
Biological and medical sciences
Case-Control Studies
Chlamydia Infections - diagnosis
Chlamydia trachomatis
Chlamydia trachomatis - isolation & purification
Etiology
Fallopian Tubes - microbiology
Female
Fundamental and applied biological sciences. Psychology
Fusobacteriaceae Infections - diagnosis
Gonorrhea - diagnosis
Human bacterial diseases
Humans
Infectious diseases
Leptotrichia
Leptotrichia - classification
Leptotrichia - isolation & purification
Medical sciences
Microbiology
Middle Aged
Molecular Sequence Data
Neisseria gonorrhoeae
Neisseria gonorrhoeae - isolation & purification
Pelvic inflammatory disease
Peptostreptococcus
Phylogeny
Polymerase chain reaction
Polymerase Chain Reaction - methods
Ribosomal DNA
RNA, Bacterial
RNA, Ribosomal, 16S
Salpingitis
Salpingitis - diagnosis
Salpingitis - microbiology
Sequence Homology, Nucleic Acid
Specimens
Streptococcus pyogenes
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Summary:Although Chlamydia trachomatis and Neisseria gonorrhoeae are established causes of salpingitis, the majority of cases have no known etiology. We used broad-range 16S rDNA polymerase chain reaction to identify novel, possibly uncultivable, bacteria associated with salpingitis and identified bacterial 16S sequences in Fallopiantube specimens from 11 (24%) of 45 consecutive women with laparoscopically confirmed acute salpingitis (the case patients) and from 0 of 44 women seeking tubal ligations (the control subjects) at Kenyatta National Hospital, Nairobi, Kenya. Bacterial phylotypes most closely related to Leptotrichia spp. were detected as the sole phylotypes in 1, and mixed with other bacterial phylotypes in 2, specimens. Novel bacterial phylotypes and those associated with bacterial vaginosis, including Atopobium vaginae, were identified in 3 specimens. N. gonorrhoeae and Streptococcus pyogenes were identified in 2 and 1 specimens, respectively. The finding of novel phylotypes associated with salpingitis has important implications for the etiology, pathogenesis, and treatment of this important reproductive-tract disease syndrome.
ISSN:0022-1899
1537-6613
DOI:10.1086/425929