Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedl...

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Bibliographic Details
Published in:Theoretical and applied genetics 2004-11, Vol.109 (8), p.1702-1709
Main Authors: Gygax, M, Gianfranceschi, L, Liebhard, R, Kellerhals, M, Gessler, C, Patocchi, A
Format: Article
Language:English
Subjects:
Analysis
Apple
Ascomycota
Biological and medical sciences
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Crop diseases
Decapoda
disease resistance
DNA Primers
Fundamental and applied biological sciences. Psychology
fungal diseases of plants
gene segregation
Genes
Genes, Plant - genetics
Genetic aspects
Genetic markers
Genetic Markers - genetics
Genetic research
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genomics
Immunity, Innate - genetics
Malus - genetics
Malus baccata
microsatellite repeats
Plant Diseases - genetics
Plant Diseases - microbiology
plant pathogenic fungi
Pteridophyta, spermatophyta
Random Amplified Polymorphic DNA Technique
scab diseases
sequence characterized amplified region
Vbj gene
Vegetals
Venturia inaequalis
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Summary:Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance gene Vbj originating from the crab apple Malus baccata jackii. Using bulk segregant analysis, three RAPD markers linked to Vbj were first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be co-dominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype-phenotype incongruence (GPI plants) plants, a linkage map was calculated. Vbj mapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6 cM from CH05e03 and at 3.9 cM from T6-SCAR. Without the removal of the GPI plants, Vbj was placed 15 cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-004-1803-9