Acid excreting mutants of yeast Saccharomyces cerevisiae

Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-12, Vol.325 (3), p.1030-1036
Main Authors: Machnicka, B., Grochowalska, R., Boniewska-Bernacka, E., Słomińska, L., Lachowicz, T.M.
Format: Article
Language:English
Subjects:
Acids - metabolism
Acids secretion
Citric Acid Cycle - physiology
Hydrogen-Ion Concentration
Krebs cycle
Mutation
Oxidation-Reduction
Protons
Respiratory deficient mutants
Saccharomyces cerevisiae
Saccharomyces cerevisiae - classification
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - growth & development
Saccharomyces cerevisiae - metabolism
Water - metabolism
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Description
Summary:Saccharomyces cerevisiae mutants acidifying glucose medium containing bromocresol purple were shown to excrete protons when placed in unbuffered water in the absence of any external carbon source. The mutants belong to 16 different complementation groups. Most of them do not grow on glycerol and the excreted protons are associated to particular sets of organic anions such as citrate, aconitate, succinate, fumarate or malate. These novel types of respiratory mutations seem to be located in genes operating in the Krebs or glyoxylate cycle.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.10.130