Mesenchymal cells: a vehicle for gene therapy

T cell-depleted allogeneic stem cell transplantation is associated with delayed immunological reconstitution. Bone marrow stroma and interleukin 7 (IL-7) regulate homeostasis of T lymphocytes. We engineered human stromal cells with a retroviral vector containing the IL-7 gene and studied in vitro ef...

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Bibliographic Details
Published in:Blood cells, molecules, & diseases molecules, & diseases, 2004-11, Vol.33 (3), p.267-270
Main Authors: Di Ianni, M., Del Papa, B., De Ioanni, M., Sportoletti, P., Moretti, L., Falzetti, F., Martelli, M.F., Tabilio, A.
Format: Article
Language:English
Subjects:
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cell Differentiation - genetics
Cell Differentiation - physiology
Cell Proliferation
Cells, Cultured
Genetic Therapy
Humans
Interleukin 7
Interleukin-7 - biosynthesis
Interleukin-7 - genetics
Lymphopoiesis
Mesoderm - cytology
Mesoderm - physiology
Stromal Cells - physiology
T cell-depleted allogeneic stem cell transplantation
T-Lymphocytes - physiology
Tissue Engineering - methods
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Summary:T cell-depleted allogeneic stem cell transplantation is associated with delayed immunological reconstitution. Bone marrow stroma and interleukin 7 (IL-7) regulate homeostasis of T lymphocytes. We engineered human stromal cells with a retroviral vector containing the IL-7 gene and studied in vitro effects on T cells. Human stromal cells were successfully transduced and generated a layer that was morphologically and phenotypically normal. IL-7-engineered stromal cells conserve the biological properties of unmanipulated stromal cells. Through their production of IL-7, they enhance survival and homeostatic proliferation of naive T cells. Because of this cytokine production, they might be an ideal vehicle for gene therapy aimed at supporting lymphopoiesis in the T cell-deficient host.
ISSN:1079-9796
1096-0961
DOI:10.1016/j.bcmd.2004.08.014