New approach to study of T cellular immunity development: Parallel investigation of lymphoid organ formation and changes in immune proteasome amount in rat early ontogenesis

The expression pattern and distribution of proteasome immune subunits LMP7 and LMP2 in the developing rat spleen and liver as well as the periarterial lymphoid sheath formation were investigated. LMP7 and LMP2 were detected by immunoblotting in the spleen on the 21st embryonic day and during the fir...

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Bibliographic Details
Published in:Cellular immunology 2009, Vol.256 (1), p.47-55
Main Authors: Sharova, Natalia P., Zakharova, Liudmila A., Astakhova, Tatiana M., Karpova, Yaroslava D., Melnikova, Victoria I., Dmitrieva, Svetlana B., Lyupina, Yulia V., Erokhov, Pavel A.
Format: Article
Language:English
Subjects:
Animals
Cysteine Endopeptidases - immunology
Cysteine Endopeptidases - metabolism
Embryonic and early postnatal development
Embryonic Development - immunology
Female
Immune proteasomes
Immunity, Cellular
Liver - embryology
Liver - enzymology
Liver - growth & development
Liver - immunology
Lymphoid Tissue - embryology
Lymphoid Tissue - enzymology
Lymphoid Tissue - growth & development
Lymphoid Tissue - immunology
Multienzyme Complexes - immunology
Multienzyme Complexes - metabolism
Periarterial lymphoid sheath
Pregnancy
Proteasome Endopeptidase Complex
Rat spleen and liver
Rats
Rats, Wistar
Spleen - embryology
Spleen - enzymology
Spleen - growth & development
Spleen - immunology
T cellular immunity
T-Lymphocytes - enzymology
T-Lymphocytes - immunology
Tissue Distribution
White pulp
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Summary:The expression pattern and distribution of proteasome immune subunits LMP7 and LMP2 in the developing rat spleen and liver as well as the periarterial lymphoid sheath formation were investigated. LMP7 and LMP2 were detected by immunoblotting in the spleen on the 21st embryonic day and during the first postnatal days in equal amounts. Their levels increased by the 8th and 18th postnatal days. Double immunofluorescent labeling the spleen cells revealed LMP7 and LMP2 in T and B lymphocytes localized in the red pulp in embryogenesis. Few T lymphocytes were discovered in periarterial zones on the 8th postnatal day. T lymphocytes filled these zones and formed lymphoid sheaths by the 18–19th day. In the liver, LMP7 and LMP2 were revealed by the 17–19th postnatal day. Immunofluorescent analysis showed their presence in hepatocytes at this period. The data suggest that T cell-mediated immune response in relation to hepatocytes is possible beginning from 18th to 19th postnatal day.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2009.01.004