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What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude?
We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length ( L B), at 0.5 × 10 −2 N] a...
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| Published in: | Respiratory physiology & neurobiology 2004-11, Vol.144 (1), p.59-70 |
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| Main Authors: | , , , , , , , , , |
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| cites | cdi_FETCH-LOGICAL-c448t-eba48af46621ccb024e0f5254ed704f243644020cce8fd3e892ddd1b1775470f3 |
| container_end_page | 70 |
| container_issue | 1 |
| container_start_page | 59 |
| container_title | Respiratory physiology & neurobiology |
| container_volume | 144 |
| creator | Garcia, Cristiane S.N.B. Rocco, Patricia R.M. Facchinetti, Lívia D. Lassance, Roberta M. Caruso, Pedro Deheinzelin, Daniel Morales, Marcelo M. Romero, Pablo V. Faffe, Débora S. Zin, Walter A. |
| description | We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1
h under different amplitudes [1, 5 and 10% of resting length (
L
B), at 0.5 × 10
−2 N] and forces (0.25 × 10
−2, 0.5 × 10
−2 and 10
−2
N, at 5%
L
B). Resistance (
R), elastance (
E) and hysteresivity (
η) were analysed during sinusoidal oscillations at 1
Hz. After 1
h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips.
E and
R increased with augmenting force and decreased with increasing amplitude, while
η remained unaltered. PCIII mRNA expression increased significantly after 1
h of oscillation at 10
−2
N and 5%
L
B and remained unchanged for 6
h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression. |
| doi_str_mv | 10.1016/j.resp.2004.07.023 |
| format | article |
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h under different amplitudes [1, 5 and 10% of resting length (
L
B), at 0.5 × 10
−2 N] and forces (0.25 × 10
−2, 0.5 × 10
−2 and 10
−2
N, at 5%
L
B). Resistance (
R), elastance (
E) and hysteresivity (
η) were analysed during sinusoidal oscillations at 1
Hz. After 1
h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips.
E and
R increased with augmenting force and decreased with increasing amplitude, while
η remained unaltered. PCIII mRNA expression increased significantly after 1
h of oscillation at 10
−2
N and 5%
L
B and remained unchanged for 6
h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.</description><identifier>ISSN: 1569-9048</identifier><identifier>EISSN: 1878-1519</identifier><identifier>DOI: 10.1016/j.resp.2004.07.023</identifier><identifier>PMID: 15522703</identifier><language>eng</language><publisher>Amsterdarm: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biomechanical Phenomena ; Collagen Type III - genetics ; Collagen Type III - metabolism ; Extracellular matrix ; Extracellular Matrix - metabolism ; Female ; Fundamental and applied biological sciences. Psychology ; Hysteresivity ; In Vitro Techniques ; Lung - metabolism ; Mechanical forces ; Procollagen mRNA ; Rat ; Rats ; Rats, Wistar ; RNA, Messenger - metabolism ; Stress, Mechanical ; Tissue mechanics ; Vertebrates: respiratory system</subject><ispartof>Respiratory physiology & neurobiology, 2004-11, Vol.144 (1), p.59-70</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c448t-eba48af46621ccb024e0f5254ed704f243644020cce8fd3e892ddd1b1775470f3</citedby><cites>FETCH-LOGICAL-c448t-eba48af46621ccb024e0f5254ed704f243644020cce8fd3e892ddd1b1775470f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,783,787,27936,27937</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16298467$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15522703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia, Cristiane S.N.B.</creatorcontrib><creatorcontrib>Rocco, Patricia R.M.</creatorcontrib><creatorcontrib>Facchinetti, Lívia D.</creatorcontrib><creatorcontrib>Lassance, Roberta M.</creatorcontrib><creatorcontrib>Caruso, Pedro</creatorcontrib><creatorcontrib>Deheinzelin, Daniel</creatorcontrib><creatorcontrib>Morales, Marcelo M.</creatorcontrib><creatorcontrib>Romero, Pablo V.</creatorcontrib><creatorcontrib>Faffe, Débora S.</creatorcontrib><creatorcontrib>Zin, Walter A.</creatorcontrib><title>What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude?</title><title>Respiratory physiology & neurobiology</title><addtitle>Respir Physiol Neurobiol</addtitle><description>We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1
h under different amplitudes [1, 5 and 10% of resting length (
L
B), at 0.5 × 10
−2 N] and forces (0.25 × 10
−2, 0.5 × 10
−2 and 10
−2
N, at 5%
L
B). Resistance (
R), elastance (
E) and hysteresivity (
η) were analysed during sinusoidal oscillations at 1
Hz. After 1
h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips.
E and
R increased with augmenting force and decreased with increasing amplitude, while
η remained unaltered. PCIII mRNA expression increased significantly after 1
h of oscillation at 10
−2
N and 5%
L
B and remained unchanged for 6
h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomechanical Phenomena</subject><subject>Collagen Type III - genetics</subject><subject>Collagen Type III - metabolism</subject><subject>Extracellular matrix</subject><subject>Extracellular Matrix - metabolism</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hysteresivity</subject><subject>In Vitro Techniques</subject><subject>Lung - metabolism</subject><subject>Mechanical forces</subject><subject>Procollagen mRNA</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - metabolism</subject><subject>Stress, Mechanical</subject><subject>Tissue mechanics</subject><subject>Vertebrates: respiratory system</subject><issn>1569-9048</issn><issn>1878-1519</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kE1r3DAQhk1padK0f6CHokt7szuSZcsuhRBCPxZCAiWhRyFLo40Wf1VjB_bfV9tdyK2nGYZnhnmfLHvPoeDA68-7IiLNhQCQBagCRPkiO-eNanJe8fZl6qu6zVuQzVn2hmgHwBVX5evsjFeVEArK82z-_WgWFkYb0RASW_Yzss1mw-Y42anvzRZHNvy6vWI9PmFPCWX9Om7ZEohW_MJoSU8cxm616Fi3Z_bRjFv8R_opWmRTZGaY-7CsDi_fZq-86QnfnepF9vD92_31z_zm7sfm-uomt1I2S46dkY3xsq4Ft7YDIRF8JSqJToH0Qpa1lCDAWmy8K7FphXOOd1ypSirw5UX26Xg3BfmzIi16CGQxJRpxWknXKX7yUyZQHEEbJ6KIXs8xDCbuNQd98Kx3-uBZHzxrUDp5TksfTtfXbkD3vHISm4CPJ8CQNb2PZrSBnrlatI2sVeK-HrnkFp8CRk024JhUhoh20W4K__vjL_sDnCk</recordid><startdate>20041130</startdate><enddate>20041130</enddate><creator>Garcia, Cristiane S.N.B.</creator><creator>Rocco, Patricia R.M.</creator><creator>Facchinetti, Lívia D.</creator><creator>Lassance, Roberta M.</creator><creator>Caruso, Pedro</creator><creator>Deheinzelin, Daniel</creator><creator>Morales, Marcelo M.</creator><creator>Romero, Pablo V.</creator><creator>Faffe, Débora S.</creator><creator>Zin, Walter A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041130</creationdate><title>What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude?</title><author>Garcia, Cristiane S.N.B. ; Rocco, Patricia R.M. ; Facchinetti, Lívia D. ; Lassance, Roberta M. ; Caruso, Pedro ; Deheinzelin, Daniel ; Morales, Marcelo M. ; Romero, Pablo V. ; Faffe, Débora S. ; Zin, Walter A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c448t-eba48af46621ccb024e0f5254ed704f243644020cce8fd3e892ddd1b1775470f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomechanical Phenomena</topic><topic>Collagen Type III - genetics</topic><topic>Collagen Type III - metabolism</topic><topic>Extracellular matrix</topic><topic>Extracellular Matrix - metabolism</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hysteresivity</topic><topic>In Vitro Techniques</topic><topic>Lung - metabolism</topic><topic>Mechanical forces</topic><topic>Procollagen mRNA</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - metabolism</topic><topic>Stress, Mechanical</topic><topic>Tissue mechanics</topic><topic>Vertebrates: respiratory system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia, Cristiane S.N.B.</creatorcontrib><creatorcontrib>Rocco, Patricia R.M.</creatorcontrib><creatorcontrib>Facchinetti, Lívia D.</creatorcontrib><creatorcontrib>Lassance, Roberta M.</creatorcontrib><creatorcontrib>Caruso, Pedro</creatorcontrib><creatorcontrib>Deheinzelin, Daniel</creatorcontrib><creatorcontrib>Morales, Marcelo M.</creatorcontrib><creatorcontrib>Romero, Pablo V.</creatorcontrib><creatorcontrib>Faffe, Débora S.</creatorcontrib><creatorcontrib>Zin, Walter A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Respiratory physiology & neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia, Cristiane S.N.B.</au><au>Rocco, Patricia R.M.</au><au>Facchinetti, Lívia D.</au><au>Lassance, Roberta M.</au><au>Caruso, Pedro</au><au>Deheinzelin, Daniel</au><au>Morales, Marcelo M.</au><au>Romero, Pablo V.</au><au>Faffe, Débora S.</au><au>Zin, Walter A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude?</atitle><jtitle>Respiratory physiology & neurobiology</jtitle><addtitle>Respir Physiol Neurobiol</addtitle><date>2004-11-30</date><risdate>2004</risdate><volume>144</volume><issue>1</issue><spage>59</spage><epage>70</epage><pages>59-70</pages><issn>1569-9048</issn><eissn>1878-1519</eissn><abstract>We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1
h under different amplitudes [1, 5 and 10% of resting length (
L
B), at 0.5 × 10
−2 N] and forces (0.25 × 10
−2, 0.5 × 10
−2 and 10
−2
N, at 5%
L
B). Resistance (
R), elastance (
E) and hysteresivity (
η) were analysed during sinusoidal oscillations at 1
Hz. After 1
h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips.
E and
R increased with augmenting force and decreased with increasing amplitude, while
η remained unaltered. PCIII mRNA expression increased significantly after 1
h of oscillation at 10
−2
N and 5%
L
B and remained unchanged for 6
h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.</abstract><cop>Amsterdarm</cop><pub>Elsevier B.V</pub><pmid>15522703</pmid><doi>10.1016/j.resp.2004.07.023</doi><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 1569-9048 |
| ispartof | Respiratory physiology & neurobiology, 2004-11, Vol.144 (1), p.59-70 |
| issn | 1569-9048 1878-1519 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67031563 |
| source | ScienceDirect Freedom Collection |
| subjects | Animals Biological and medical sciences Biomechanical Phenomena Collagen Type III - genetics Collagen Type III - metabolism Extracellular matrix Extracellular Matrix - metabolism Female Fundamental and applied biological sciences. Psychology Hysteresivity In Vitro Techniques Lung - metabolism Mechanical forces Procollagen mRNA Rat Rats Rats, Wistar RNA, Messenger - metabolism Stress, Mechanical Tissue mechanics Vertebrates: respiratory system |
| title | What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude? |
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