What increases type III procollagen mRNA levels in lung tissue: stress induced by changes in force or amplitude?

We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length ( L B), at 0.5 × 10 −2 N] a...

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Bibliographic Details
Published in:Respiratory physiology & neurobiology 2004-11, Vol.144 (1), p.59-70
Main Authors: Garcia, Cristiane S.N.B., Rocco, Patricia R.M., Facchinetti, Lívia D., Lassance, Roberta M., Caruso, Pedro, Deheinzelin, Daniel, Morales, Marcelo M., Romero, Pablo V., Faffe, Débora S., Zin, Walter A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biomechanical Phenomena
Collagen Type III - genetics
Collagen Type III - metabolism
Extracellular matrix
Extracellular Matrix - metabolism
Female
Fundamental and applied biological sciences. Psychology
Hysteresivity
In Vitro Techniques
Lung - metabolism
Mechanical forces
Procollagen mRNA
Rat
Rats
Rats, Wistar
RNA, Messenger - metabolism
Stress, Mechanical
Tissue mechanics
Vertebrates: respiratory system
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Summary:We hypothesized that stress determined by force could induce higher type III procollagen (PCIII) mRNA expression than the stress determined by amplitude. To that end, rat lung tissue strips were oscillated for 1 h under different amplitudes [1, 5 and 10% of resting length ( L B), at 0.5 × 10 −2 N] and forces (0.25 × 10 −2, 0.5 × 10 −2 and 10 −2 N, at 5% L B). Resistance ( R), elastance ( E) and hysteresivity ( η) were analysed during sinusoidal oscillations at 1 Hz. After 1 h of oscillation, PCIII mRNA expression was determined by Northern-blot and semiquantitative RT-PCR. Control value of PCIII mRNA was obtained from unstressed strips. E and R increased with augmenting force and decreased with increasing amplitude, while η remained unaltered. PCIII mRNA expression increased significantly after 1 h of oscillation at 10 −2 N and 5% L B and remained unchanged for 6 h. In conclusion, the stress induced by force but not by amplitude led to the increment in PCIII mRNA expression.
ISSN:1569-9048
1878-1519
DOI:10.1016/j.resp.2004.07.023