In vitro cytotoxicity of maxillofacial silicone elastomers: Effect of accelerated aging

The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L‐929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc‐shaped test samples of maxillofacial silicone elas...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2009-04, Vol.89B (1), p.122-126
Main Authors: Bal, Bilge Turhan, Yılmaz, Handan, Aydın, Cemal, Karakoca, Seçil, Yılmaz, Şükran
Format: Article
Language:English
Subjects:
Aging
Animals
Biocompatible Materials - chemistry
Biocompatible Materials - pharmacology
cell culture
Cell Line - drug effects
Cell Survival - drug effects
cytotoxicity
Humans
Materials Testing
maxillofacial
Maxillofacial Prosthesis
Mice
Silicone Elastomers - chemistry
Silicone Elastomers - pharmacology
silicone(s)/PDMS
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Summary:The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L‐929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc‐shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L‐929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05). © 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.31194