Evaluation of apparent formation constants of pentacyclic triterpene acids complexes with derivatized beta- and gamma-cyclodextrins by reversed phase liquid chromatography

A reversed phase HPLC method has been investigated in order to resolve three main pentacyclic triterpene acids (oleanolic-, betulinic- and ursolic acid) found in a lot of plants. Some of them (oleanolic and ursolic acids) are position isomers and their resolution is highly improved by the addition o...

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Bibliographic Details
Published in:Journal of Chromatography A 2004-09, Vol.1049 (1-2), p.37-42
Main Authors: Claude, B, Morin, P, Lafosse, M, Andre, P
Format: Article
Language:English
Subjects:
Alicyclic compounds, terpenoids, prostaglandins, steroids
Analytical chemistry
beta-Cyclodextrins - chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid
Drug Compounding
Exact sciences and technology
gamma-Cyclodextrins - chemistry
Indicators and Reagents
Organic chemistry
Other chromatographic methods
Polycyclic Compounds - chemistry
Preparations and properties
Regression Analysis
Solubility
Spectrophotometry, Ultraviolet
Terpenoids
Triterpenes - chemistry
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Summary:A reversed phase HPLC method has been investigated in order to resolve three main pentacyclic triterpene acids (oleanolic-, betulinic- and ursolic acid) found in a lot of plants. Some of them (oleanolic and ursolic acids) are position isomers and their resolution is highly improved by the addition of derivatized cyclodextrins in mobile phase. The formation of 1:1 inclusion complexes was assumed. Apparent formation constants of triterpene acids with DM-beta-CD and HP-gamma-CD were determined by HPLC method. Experimental results confirmed the complexation model and explained the modification of elution order according to the type of cyclodextrin added to the mobile phase. The influence of mobile phase organic modifier on apparent formation constants was also investigated. Results proved the competition between cyclodextrins hydrophobic cavity and organic solvent towards triterpene acids affinity.
ISSN:0021-9673
DOI:10.1016/S0021-9673(04)01143-4