Transporter mRNA Expression in a Conditionally Immortalized Rat Small Intestine Epithelial Cell Line (TR-SIE)

Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a pol...

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Published in:DRUG METABOLISM AND PHARMACOKINETICS 2004, Vol.19 (4), p.264-269
Main Authors: Hosoya, Ken-ichi, Tomi, Masatoshi, Takayama, Megumi, Komokata, Yuko, Nakai, Daisuke, Tokui, Taro, Nishimura, Kenji, Ueda, Masatsugu, Obinata, Masuo, Hori, Satoko, Ohtsuki, Sumio, Amidon, Gordon L., Terasaki, Tetsuya
Format: Article
Language:eng ; jpn
Subjects:
Animals
Cell Line, Transformed
Cells, Cultured
Epithelial Cells - cytology
Epithelial Cells - metabolism
Intestine, Small - cytology
Intestine, Small - metabolism
Male
Membrane Transport Proteins - biosynthesis
Membrane Transport Proteins - genetics
Rats
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
small intestine epithelial cells
SV 40 large T-antigen
transporter
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Summary:Small intestine epithelial cell lines (TR-SIE), which are established from the small intestine of transgenic rats harboring temperature-sensitive simian virus 40 large T-antigen gene (tsA58 Tg rat), were used to characterize the mRNA expression of small intestine transporters. TR-SIE cells had a polygonal morphology and expressed cytokeratin protein and villin mRNA. Although the large T-antigen was strongly expressed at 33°C, this was reduced at 37 and 39°C. Concomitantly, the cell growth was arrested at 37 and 39°C compared with that at 33°C, suggesting that TR-SIE cells are conditionally immortalized cell lines. RT-PCR analysis revealed that TR-SIE cells expressed ABCB1 (mdr1a and mdr1b), ABCB4 (mdr2), ABCC2 (mrp2), ABCC6 (mrp6), ABCG1, ABCG2 (bcrp/mxr), Slc21a7 (Oatp3), Slc15a1 (PepT1), and Slc16a1 (Mct1). Conditionally immortalized rat small intestine epithelial cell lines were established from tsA58 Tg rats and expressed the mRNA of intestinal transporters
ISSN:1347-4367
1880-0920
DOI:10.2133/dmpk.19.264