Mouse transcriptome Neutral evolution of 'non-coding' complementary DNAs

(Arising from: Y. Okazaki et al. Nature 420, 563-573 200210.1038/nature01266; Okazaki et al. reply)Okazaki et al. have argued that as many as 15,815 of 33,409 non-redundant mouse complementary DNAs may represent functional RNA genes, on the basis of their findings that some of these cDNAs are confir...

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Published in:	Nature 2004-10, Vol.431 (7010), p.1 p following 757; discussion following 757-1 p following 757; discussion following 757
Main Authors:	Wong, Gane Ka-Shu, Wang, Jun, Zhang, Jianguo, Zheng, Hongkun, Li, Jun, Liu, Dongyuan, Li, Heng, Samudrala, Ram, Yu, Jun
Format:	Article
Language:	eng
Subjects:	Animals Computational Biology Conserved Sequence - genetics CpG Islands - genetics Databases, Genetic DNA, Complementary - genetics DNA, Intergenic - genetics Evolution, Molecular Expressed Sequence Tags Genomics Humans Mice Models, Genetic Rats Reproducibility of Results RNA, Untranslated - genetics Species Specificity Transcription, Genetic - genetics
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Summary:	(Arising from: Y. Okazaki et al. Nature 420, 563-573 200210.1038/nature01266; Okazaki et al. reply)Okazaki et al. have argued that as many as 15,815 of 33,409 non-redundant mouse complementary DNAs may represent functional RNA genes, on the basis of their findings that some of these cDNAs are confirmed by expressed sequence tagging and are found near CpG islands or polyadenylation signals - although many are expressed at such low levels that they could not be detected by microarray analysis. We show here that conservation of these 'non-coding' cDNAs in rats or humans is no better than in an evolutionarily neutral control. Our results indicate that they are either non-functional or, if they are functional, are specific to a given species.
ISSN:	0028-0836 1476-4687