Collisional Activation of Protein Complexes: Picking Up the Pieces

Mass spectrometry is fast becoming a vital approach not only for the identification and quantification of proteins, but also for the study of the noncovalent assemblies they form. Approaches for ionizing, transmitting, and detecting protein complexes intact in the mass spectrometer are now well esta...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2009-03, Vol.20 (3), p.341-348
Main Author: Benesch, Justin L.P.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Analytical, structural and metabolic biochemistry
Assemblies
Bioinformatics
Biological and medical sciences
Biotechnology
Chemistry
Chemistry and Materials Science
Critical Insight
Dismantling
Escherichia coli
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Ionic mobility
Ions
Mass spectrometry
Mass Spectrometry - methods
Multiprotein Complexes - chemistry
Organic Chemistry
Peptide Fragments - chemistry
Proteins
Proteins - chemistry
Proteomics
Recombinant Proteins - chemistry
Scientific imaging
Spectroscopy
Thermodynamics
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Summary:Mass spectrometry is fast becoming a vital approach not only for the identification and quantification of proteins, but also for the study of the noncovalent assemblies they form. Approaches for ionizing, transmitting, and detecting protein complexes intact in the mass spectrometer are now well established. The challenge has therefore shifted to developing and applying mass spectrometry approaches to elucidate the structure of such species. A crucial aspect to this goal is inducing their disassembly in the gas phase to mine information as to their composition and organization. Here the consequences of collisionally activating protein complexes are illustrated through ion mobility mass spectrometry measurements and discussed in the context of the current literature. Although a consensus view of the mechanism of dissociation is starting to emerge, it is also clear that a number of aspects remain unresolved. These outstanding questions and frontier challenges must be addressed if gas-phase dissociative approaches are to reach their full potential in the study of protein assemblies. The mechanism by which protein complexes dissociate in the gas phase is becoming clearer, but a number of outstanding questions remain to be answered!
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2008.11.014