Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery

We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic...

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Published in:European journal of pharmacology 2009-03, Vol.605 (1), p.129-137
Main Authors: Seto, Sai Wang, Lam, Ho Yeung, Lau, Wing Sze, Au, Alice L.S., Lam, Tsz Yan, Chim, Stephen S.C., Ngai, Sai Ming, Chan, Shun Wan, Leung, Tak Yeung, Yeung, John H.K., Kong, Siu Kai, Leung, George P.H., Lee, Simon M.Y., Kwan, Yiu Wa
Format: Article
Language:English
Subjects:
5-hydroxytryptamine
Adult
Biological and medical sciences
Caveolin 1 - metabolism
Diseases of mother, fetus and pregnancy
Dose-Response Relationship, Drug
Female
Gene Expression Regulation, Enzymologic
Gynecology. Andrology. Obstetrics
Humans
Isometric Contraction
Medical sciences
Monoamine oxidase
Monoamine Oxidase - genetics
Monoamine Oxidase - metabolism
Nitric Oxide Synthase Type III - genetics
Nitric Oxide Synthase Type III - metabolism
Pharmacology. Drug treatments
Pre-Eclampsia - metabolism
Preeclampsia
Pregnancy
Pregnancy. Fetus. Placenta
Receptors, Serotonin - metabolism
Serotonin - administration & dosage
Serotonin - metabolism
Serotonin Plasma Membrane Transport Proteins - metabolism
Umbilical Arteries - metabolism
Umbilical artery
Young Adult
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Summary:We investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) ( N ω-nitro- l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT 2B, SB 204741; 5-HT 2C, RS 102221; 5-HT 7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development.
ISSN:0014-2999
1879-0712
DOI:10.1016/j.ejphar.2008.12.054