Inhibitory effects of Agaricus blazei extracts on human myeloid leukemia cells

Agaricus blazei has been used as an adjuvant in cancer chemotherapy and is found to inhibit the growth of various types of tumor cells. Our study has adopted a systematic and bioassay-guided approach to optimize the extraction of Agaricus blazei for anti-leukemic bioactive components. The tumor-sele...

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Bibliographic Details
Published in:Journal of ethnopharmacology 2009-03, Vol.122 (2), p.320-326
Main Authors: Kim, Chi-Fai, Jiang, Jing-Jing, Leung, Kwok-Nam, Fung, Kwok-Pui, Lau, Clara Bik-San
Format: Article
Language:English
Subjects:
adjuvants
Agaricus
Agaricus blazei
animal disease models
Animals
Anti-tumor
anticarcinogenic activity
Antineoplastic Agents - pharmacology
Antineoplastic Agents - therapeutic use
Apoptosis
Apoptosis - drug effects
bioassays
Biological and medical sciences
Cancer
Cell Line
Cell Line, Tumor
Cytotoxic
cytotoxicity
DNA fragmentation
functional foods
General pharmacology
human cell lines
Humans
in vitro studies
in vivo studies
Leukemia
Leukemia, Myeloid - drug therapy
Liver - cytology
Liver - drug effects
Medical sciences
Mice
Mice, Nude
mushrooms
myeloid leukemia
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Phytotherapy
plant extracts
Plant Extracts - pharmacology
Plant Extracts - therapeutic use
polysaccharides
Polysaccharides - pharmacology
Polysaccharides - therapeutic use
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Summary:Agaricus blazei has been used as an adjuvant in cancer chemotherapy and is found to inhibit the growth of various types of tumor cells. Our study has adopted a systematic and bioassay-guided approach to optimize the extraction of Agaricus blazei for anti-leukemic bioactive components. The tumor-selective growth inhibitory activity of the extracts on leukemic cell lines was evaluated in vitro and in vivo using tumor-bearing nude mice. Agaricus blazei extracts were prepared using different methods. MTT and tritiated thymidine incorporation assays were used to evaluate the in vitro anti-leukemic effects. The most potent extract was further investigated using NB-4 cells-bearing nude mice and mechanistic studies using DNA fragmentation assay and cell death detection ELISA. The JAB80E70 extract showed the most potent tumor-selective growth inhibitory activity against human leukemia NB-4 and K-562 cells. This is the first report of anti-leukemic activity of JAB80E70 in athymic nude mice bearing NB-4 cells. Using DNA fragmentation assays and cell death detection ELISA, JAB80E70 was found to induce apoptosis in NB-4 cells. However, the polysaccharide enriched fractions failed to show significant cytotoxicity on NB-4 cells in vitro. The JAB80E70 extract exhibited potent anti-leukemic effect in vitro and in vivo. The effect can be attributed, at least in part, to the induction of apoptosis. Besides, polysaccharides in Agaricus blazei may not possess direct anti-leukemic activity in vitro.
ISSN:0378-8741
1872-7573
DOI:10.1016/j.jep.2008.12.025