An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibrobla...

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Bibliographic Details
Published in:Fertility and sterility 2004-10, Vol.82 (4), p.960-962
Main Authors: Chang, Kyung H., Lim, Jeong M., Kang, Sung K., Lee, Byeong C., Moon, Shin Y., Hwang, Woo S.
Format: Article
Language:English
Subjects:
Animals
Cattle - embryology
Cell Culture Techniques
Cell Fusion - methods
Culture Media
Female
Fibroblasts - cytology
Gene Transfer, Horizontal - genetics
Humans
Mammary Glands, Human - cytology
Oocytes - cytology
Prospective Studies
Random Allocation
Umbilical Cord - cytology
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Summary:Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca 2+/Mg 2+-free fusion medium and a serum-free medium being used for bovine embryos.
ISSN:0015-0282
1556-5653
DOI:10.1016/j.fertnstert.2004.03.052