α-Eleostearic Acid (9Z11E13E-18:3) Is Quickly Converted to Conjugated Linoleic Acid (9Z11E-18:2) in Rats

We previously showed that α-eleostearic acid (α-ESA; 9Z11E13E-18:3) is converted to conjugated linoleic acid (CLA; 9,11–18:2) in the liver and plasma of rats that were given diets including 1% α-ESA for 4 wk. In this study, we investigated this phenomenon in detail. First, the chemical structure of...

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Bibliographic Details
Published in:The Journal of nutrition 2004-10, Vol.134 (10), p.2634-2639
Main Authors: Miyazawa, Teruo, Tsuzuki, Tsuyoshi, Tokuyama, Yoshiko, Igarashi, Miki, Nakagawa, Kiyotaka, Ohsaki, Yusuke, Komai, Michio
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid
conjugated linoleic acid
conjugated linolenic acid
eleostearic acid
Fundamental and applied biological sciences. Psychology
GC-EI/MS
germ-free rat
Linoleic Acids, Conjugated - blood
Linoleic Acids, Conjugated - metabolism
Linolenic Acids - metabolism
Lipids. Glycolipids
Liver - metabolism
Male
Metabolisms and neurohumoral controls
Rats
Rats, Sprague-Dawley
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:We previously showed that α-eleostearic acid (α-ESA; 9Z11E13E-18:3) is converted to conjugated linoleic acid (CLA; 9,11–18:2) in the liver and plasma of rats that were given diets including 1% α-ESA for 4 wk. In this study, we investigated this phenomenon in detail. First, the chemical structure of CLA produced by α-ESA administration was determined. After α-ESA was orally administered to rats, CLA in rat liver was isolated by HPLC. The positional and geometric isomerism was determined using GC-EI/MS and 13C-NMR, respectively, and the CLA generated in rats after α-ESA feeding was confirmed to be 9Z11E-CLA. Next, the concentrations of α-ESA and CLA were determined 0, 3, 6, and 24 h after oral administration of α-ESA to rats. Moreover, we also investigated whether enteric bacteria are involved in the conversion of α-ESA to CLA using germ-free rats. α-ESA was orally administered to germ-free and normal rats and α-ESA and CLA were detected in the organs of both groups. In addition, to confirm that this reaction was enzyme-mediated, α-ESA was reacted with tissue homogenates (liver, kidney, and small intestine mucous) and coenzymes (NADH, NAD+, NADPH, and NADP+), and the enzyme activities were estimated from the amount of CLA produced. CLA was detected when α-ESA was reacted with liver, kidney, and small intestine mucous homogenates and a coenzyme (NADPH). These results indicated that α-ESA is converted to 9Z11E-CLA in rats by a Δ13-saturation reaction carried out by an NADPH-dependent enzyme.
ISSN:0022-3166
1541-6100
DOI:10.1093/jn/134.10.2634