Peri/nuclear localization of intact insulin-like growth factor binding protein-2 and a distinct carboxyl-terminal IGFBP-2 fragment in vivo

Insulin-like growth factor binding protein-2 (IGFBP-2) as one of the most important IGFBPs has never been assessed in the intracellular compartment in vivo. Since there is evidence for novel intracellular functions of distinct IGFBPs, we investigated the presence of IGFBP-2 inside the cell. In peri/...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2004-11, Vol.324 (2), p.705-710
Main Authors: Hoeflich, A., Reisinger, R., Schuett, B.S., Elmlinger, M.W., Russo, V.C., Vargas, G.A., Jehle, P.M., Lahm, H., Renner-Müller, I., Wolf, E.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Blotting, Western
Cell Nucleus - metabolism
Centrifugation, Density Gradient
IGFBP-2 fragments
Immunoprecipitation
Insulin-Like Growth Factor Binding Protein 2 - chemistry
Insulin-Like Growth Factor Binding Protein 2 - metabolism
Insulin-like growth factor binding protein-2
Ligands
Mice
Mice, Transgenic
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Overexpression
Peptides - chemistry
Peri/nuclear localization
Propidium - pharmacology
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Tissue Distribution
Transgenic mice
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Insulin-like growth factor binding protein-2 (IGFBP-2) as one of the most important IGFBPs has never been assessed in the intracellular compartment in vivo. Since there is evidence for novel intracellular functions of distinct IGFBPs, we investigated the presence of IGFBP-2 inside the cell. In peri/nuclear fractions of various tissues isolated from IGFBP-2 transgenic and non-transgenic mice we were able to show the presence of intact IGFBP-2. In addition, we demonstrate the presence of a highly conserved carboxyl-terminal IGFBP-2 fragment in the peri/nuclear fraction by using different peptide-induced antibodies. In pancreatic sections, confocal microscopy revealed the presence of IGFBP-2 on the nuclear surface but not within the nucleus. Our findings suggest novel functions of intact IGFBP-2 and IGFBP-2 fragments within the cell.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2004.09.111