Antigenic Activity of Three Chimeric Synthetic Peptides of the Transmembrane (Gp41) and the Envelope (Gp120) Glycoproteins of HIV-1 Virus

The antigenicity of three chimeric synthetic peptides (Qm, Qm-16, and Qm-17) incorporating an immunodominant epitope of the gp41 transmembrane protein (587-617) and the different epitopes of the gp120 envelope protein (495-516), (301-335), (502-516) of human immunodeficiency virus (HIV-1), separated...

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Bibliographic Details
Published in:Preparative biochemistry & biotechnology 2004-08, Vol.34 (3), p.227-237
Main Authors: Marin, Milenen Hernández, Peña, Lilliam Pozo, Tanty, Chryslaine Rodríguez, Clarke, David Higginson, Arenas, Martha Amat, Noguerol, Kenia Ruenes, León, Carlos Silva
Format: Article
Language:English
Subjects:
Chimeric synthetic peptides
Enzyme-Linked Immunosorbent Assay
gp120
gp41
HIV Antibodies - blood
HIV Antibodies - immunology
HIV Envelope Protein gp120 - chemistry
HIV Envelope Protein gp120 - immunology
HIV Envelope Protein gp41 - chemistry
HIV Envelope Protein gp41 - immunology
HIV Infections - blood
HIV-1
HIV-1 - chemistry
HIV-1 - immunology
Humans
Peptides - chemistry
Peptides - immunology
UMELISA
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Summary:The antigenicity of three chimeric synthetic peptides (Qm, Qm-16, and Qm-17) incorporating an immunodominant epitope of the gp41 transmembrane protein (587-617) and the different epitopes of the gp120 envelope protein (495-516), (301-335), (502-516) of human immunodeficiency virus (HIV-1), separated by two glycine residues, was evaluated by UltramicroEnzyme-linked immunosorbent assay (UMELISA) by using panels of anti-HIV-1 positive sera (n = 47). The specificity was evaluated with samples from healthy blood donors (n = 20) and anti-HIV-2 positive samples (n = 10). The results indicate that the chimeric peptide, Qm, was the most reactive one because it detected antibodies to virus efficiently. This may be related to peptide adsorption onto the solid surface, the C-terminal region of HIV-1 gp120 (495-516) combined with gp41 (587-617) in the chimera, and the epitope accessibility to the antibodies. This study showed the usefulness of the chimeric peptides as antigen to detect antibodies to HIV-1 virus.
ISSN:1082-6068
1532-2297
DOI:10.1081/PB-200026804