Comparison of the mycobacteria growth indicator tube with solid culture for the detection of tuberculosis complex mycobacteria from blood

The aim of this study was to compare the mycobacteria growth indicator tube and solid culture for recovery of complex tuberculosis mycobacteria from blood. One hundred and twenty-five specimens from 67 Djiboutian patients with a positive serologic diagnosis of HIV and fever were collected in an Isol...

Full description

Saved in:
Bibliographic Details
Published in:Pathologie biologie (Paris) 2009-02, Vol.57 (1), p.44-50
Main Authors: Gérôme, P, Fabre, M, Soler, C-P, De Pina, J J, Simon, F
Format: Article
Language:fre
Subjects:
Adult
Bacteremia - complications
Bacteremia - microbiology
Bacteriological Techniques - instrumentation
Bacteriological Techniques - methods
Blood - microbiology
Cerebrospinal Fluid - microbiology
Culture Media
Djibouti - epidemiology
Female
Fever - etiology
Gastrointestinal Contents - microbiology
HIV Infections - blood
HIV Infections - complications
HIV Infections - epidemiology
Humans
Male
Mycobacterium - growth & development
Mycobacterium - isolation & purification
Mycobacterium Infections - blood
Mycobacterium Infections - complications
Mycobacterium Infections - epidemiology
Mycobacterium Infections - microbiology
Mycobacterium tuberculosis - growth & development
Mycobacterium tuberculosis - isolation & purification
Prevalence
Prospective Studies
Sensitivity and Specificity
Tuberculosis - blood
Tuberculosis - complications
Tuberculosis - epidemiology
Tuberculosis - microbiology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this study was to compare the mycobacteria growth indicator tube and solid culture for recovery of complex tuberculosis mycobacteria from blood. One hundred and twenty-five specimens from 67 Djiboutian patients with a positive serologic diagnosis of HIV and fever were collected in an Isolator tube. After centrifugation and washing with phosphate-buffer, smears were prepared from the pellet for auramin staining. The remaining sediment was suspended in 1 ml of buffer. One half was inoculated into two MGIT (incubation at 30 and 37 degrees C into Bactec 960) and the other onto two Loewenstein-Jensen and two Coletsos medium (incubation at 30 and 37 degrees C). Eight cultures were contaminated: three on solid medium and MGIT simultaneously, five in MGIT only (three coagulase negative staphylococci, five enterobacteria). Fourteen strains of M. tuberculosis (six patients) and three M. canettii (two patients) (12 on solid media and MGIT, five in MGIT only) were recovered. The mean time to detection was 32.8 days for solid medium and 20.4 days for MGIT. Of a total of 25 patients with culture-proven tuberculosis, two patients had a positive blood culture only, six had blood and other specimens positive culture, 17 had a non blood specimens positive culture only. MGIT processed into Bactec 960 is a viable tool for the detection of complexe tuberculosis mycobacteria from blood and the high-frequency of these mycobacteremia in HIV infected patients from country where the prevalence of tuberculosis is high is confirmed. However, the cost/benefit ratio of this bacteriologic diagnosis had to be evaluated in developping country.
ISSN:0369-8114
1768-3114
DOI:10.1016/j.patbio.2008.05.007