Ultra-performance liquid chromatography coupled to quadrupole-orthogonal time-of-flight mass spectrometry

Ultra‐performance liquid chromatography (UPLC) utilizes sub‐2 μm particles with high linear solvent velocities to effect dramatic increases in resolution, sensitivity and speed of analysis. The reduction in particle size to below 2 μm requires instrumentation that can operate at pressures in the 600...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2004-01, Vol.18 (19), p.2331-2337
Main Authors: Plumb, Robert, Castro-Perez, Jose, Granger, Jennifer, Beattie, Iain, Joncour, Karine, Wright, Andrew
Format: Article
Language:English
Subjects:
Animals
Bile - chemistry
Chromatography, High Pressure Liquid - methods
Microchemistry - methods
Midazolam - analysis
Nanotechnology - methods
Particle Size
Rats
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
Systems Integration
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Summary:Ultra‐performance liquid chromatography (UPLC) utilizes sub‐2 μm particles with high linear solvent velocities to effect dramatic increases in resolution, sensitivity and speed of analysis. The reduction in particle size to below 2 μm requires instrumentation that can operate at pressures in the 6000–15 000 psi range. The typical peak widths generated by the UPLC system are in the order of 1–2 s for a 10‐min separation. In the present work this technology has been applied to the study of in vivo drug metabolism, in particular the analysis of drug metabolites in bile. The reduction in peak width significantly increases analytical sensitivity by three‐ to five‐fold, and the reduction in peak width, and concomitant increase in peak capacity, significantly reduces spectral overlap resulting in superior spectral quality in both MS and MS/MS modes. The application of UPLC/MS resulted in the detection of additional drug metabolites, superior separation and improved spectral quality. Copyright © 2004 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/rcm.1627