Over-expression of STP13, a hexose transporter, improves plant growth and nitrogen use in Arabidopsis thaliana seedlings

In Arabidopsis thaliana, the regulation of hexose levels by the large monosaccharide transporter (MST) gene family influences many aspects of plant growth. The cloning and transgenic expression of one family member (STP13) enabled the manipulation of carbon (C) and nitrogen (N) metabolism in Arabido...

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Bibliographic Details
Published in:Plant, cell and environment cell and environment, 2009-03, Vol.32 (3), p.271-285
Main Authors: SCHOFIELD, R.A, BI, Y.-M, KANT, S, ROTHSTEIN, S.J
Format: Article
Language:English
Subjects:
Anion Transport Proteins - metabolism
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Biological and medical sciences
Biomass
carbon
Carbon - metabolism
Cells, Cultured
Cloning, Molecular
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
glucose
Glucose - metabolism
Monosaccharide Transport Proteins - genetics
Monosaccharide Transport Proteins - metabolism
monosaccharide transporter
Nicotiana - metabolism
nitrate
Nitrogen - metabolism
Plants, Genetically Modified - genetics
Plants, Genetically Modified - growth & development
Plants, Genetically Modified - metabolism
Promoter Regions, Genetic
RNA, Plant - genetics
Seedlings - genetics
Seedlings - growth & development
Seedlings - metabolism
Sucrose - metabolism
Symporters - genetics
Symporters - metabolism
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Summary:In Arabidopsis thaliana, the regulation of hexose levels by the large monosaccharide transporter (MST) gene family influences many aspects of plant growth. The cloning and transgenic expression of one family member (STP13) enabled the manipulation of carbon (C) and nitrogen (N) metabolism in Arabidopsis. Transgenic seedlings constitutively over-expressing STP13 (STP13OX) had increased rates of glucose uptake, higher endogenous sucrose levels and accumulated more total C and biomass per plant when grown on soil-less media supplemented with 55 m m glucose and sufficient N (9 m m nitrate). Furthermore, STP13OX seedlings acquired 90% more total N than the Col-0 seedlings, and had higher levels of expression of the nitrate transporter NRT2.2. In addition, STP13OX seedlings were larger and had higher biomass than Col-0 seedlings when grown under a limiting N condition (3 m m nitrate). Transgene analysis of STP13 reveals that its gene product is localized to the plasma membrane (PM) in tobacco BY-2 suspension cells, that it encodes a functional MST in planta, and that the STP13 promoter directs GUS expression to the vasculature and to leaf mesophyll cells. This work highlights the link between C and N metabolism, demonstrating that a plant's N use may be improved by increasing the availability of C.
ISSN:0140-7791
1365-3040
DOI:10.1111/j.1365-3040.2008.01919.x