A novel model of wound healing in the SCID mouse using a cultured human skin substitute

SUMMARY Studies of skin graft behaviour in rodent excisional wound models are limited by the dominance of wound contracture and graft sloughing as primary healing responses. To slow skin contraction, polytetrafluoroethylene (Teflon) rings were inserted into dorso‐lateral full‐thickness wounds in SCI...

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Published in:Australasian journal of dermatology 2009-02, Vol.50 (1), p.29-35
Main Authors: Windsor, Martin L, Eisenberg, Mark, Gordon-Thomson, Clare, Moore, Geoffrey PM
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cells, Cultured
Collagen
cultured skin substitute
Dermatologic Surgical Procedures
Dermatology
Disease Models, Animal
Fibroblasts
Humans
Keratinocytes
Medical sciences
Mice
Mice, SCID
OrCel
Polytetrafluoroethylene
SCID mouse
Skin - injuries
skin graft
Skin Transplantation - methods
Skin, Artificial
Transplantation, Heterologous - methods
wound contraction
Wound Healing
wound-healing model
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Summary:SUMMARY Studies of skin graft behaviour in rodent excisional wound models are limited by the dominance of wound contracture and graft sloughing as primary healing responses. To slow skin contraction, polytetrafluoroethylene (Teflon) rings were inserted into dorso‐lateral full‐thickness wounds in SCID mice. Cultured skin substitutes (OrCel), composed of cultured human keratinocytes and fibroblasts in a bovine collagen sponge, were implanted within the rings. Examination and histology of grafts 14 days later showed graft take in four of six recipients, with 90% epithelialization and wound contraction of 31–47%. Immunohistochemical studies, using human‐specific antisera to distinguish graft from host tissues, showed that regenerated tissue was predominantly human. Staining with anticytokeratin, revealed a multilayered, stratified neoepidermis. HBG were identified in keratinocytes in all epidermal layers. Langerhans cells were absent. Antihuman vimentin, used as a fibroblast marker, confirmed that cells of the neodermis were primarily of human origin. Neoepidermal keratinocytes, primarily in the basal and suprabasal layers, were also stained. Results suggest that the poly(tetrafluoroethylene) ring inhibited graft sloughing and provided a more favourable environment for the skin substitute to regenerate a substantially normal human skin.
ISSN:0004-8380
1440-0960
DOI:10.1111/j.1440-0960.2008.00512.x