Association of Blastocystis subtype 3 and 1 with patients from an Oregon community presenting with chronic gastrointestinal illness

Chronic gastrointestinal illness of unknown etiology is a significant problem in the United States. Using a real-time LightCycler PCR assay we detected Blastocystis in nine patients from a metropolitan area of Corvallis, Oregon who presented with diarrhea, abdominal pain, fatigue, joint pain, skin r...

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Bibliographic Details
Published in:	Parasitology research (1987) 2009, Vol.104 (2), p.341-345
Main Authors:	Jones, Morris S, Whipps, Christopher M, Ganac, Robert D, Hudson, N. Ryan, Boroom, Kenneth
Format:	Article
Language:	eng
Subjects:	Adult Animals Biological and medical sciences Biomedical and Life Sciences Biomedicine Blastocystis - classification Blastocystis - genetics Blastocystis - isolation & purification Blastocystis Infections - epidemiology Blastocystis Infections - parasitology Child DNA, Protozoan - chemistry DNA, Protozoan - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Female Fundamental and applied biological sciences. Psychology Gastroenteritis - epidemiology Gastroenteritis - parasitology General aspects General aspects and techniques. Study of several systematic groups. Models Genotype Humans Immunology Infant Invertebrates Male Medical Microbiology Microbiology Middle Aged Oregon - epidemiology Original Paper Phylogeny Polymerase Chain Reaction - methods RNA, Ribosomal, 18S - genetics Sequence Analysis, DNA
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Summary:	Chronic gastrointestinal illness of unknown etiology is a significant problem in the United States. Using a real-time LightCycler PCR assay we detected Blastocystis in nine patients from a metropolitan area of Corvallis, Oregon who presented with diarrhea, abdominal pain, fatigue, joint pain, skin rash and psychiatric co-morbidity. Phylogenetic analysis identified six infections with Blastocystis sp. subtype 3, and one with subtype 1, using the standard Stensvold nomenclature. Most patients with subtype 3 had previously tested negative with conventional parasitological diagnostics, had been symptomatic for over 4 years, and reported antibiotic failure.
ISSN:	0932-0113 1432-1955