Sequential injection chromatographic determination of paracetamol, caffeine, and acetylsalicylic acid in pharmaceutical tablets

In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed‐phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith® Flash RP‐...

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Bibliographic Details
Published in:Journal of separation science 2004-05, Vol.27 (7-8), p.529-536
Main Authors: Šatínský, Dalibor, Neto, Isabel, Solich, Petr, Sklenářová, Hana, Conceição, M., Montenegro, B. S. M., Araújo, Alberto N.
Format: Article
Language:English
Subjects:
Acetaminophen - analysis
Acetylsalicylic acid
Analgesics
Analysis
Analytical biochemistry: general aspects, technics, instrumentation
Analytical chemistry
Analytical, structural and metabolic biochemistry
Aspirin - analysis
Biological and medical sciences
Caffeine
Caffeine - analysis
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography - methods
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General pharmacology
Indicators and Reagents
Medical sciences
Monolithic columns
Neuropharmacology
Paracetamol
Pharmacology. Drug treatments
Sensitivity and Specificity
Sequential injection analysis (SIA)
Sequential injection chromatography (SIC)
Solvents
Spectrophotometry, Ultraviolet - methods
Tablets - chemistry
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Summary:In this contribution, a new separation method for simultaneous determination of paracetamol, caffeine, acetylsalicylic acid, and internal standard benzoic acid was developed based on a novel reversed‐phase sequential injection chromatography (SIC) technique with UV detection. A Chromolith® Flash RP‐18e, 25‐4.6 mm column (Merck, Germany) and a FIAlab® 3000 system (USA) with an 8‐port selection valve and a 5 mL syringe were used for sequential injection chromatographic separations in our study. The mobile phase used was acetonitrile‐(0.01 M) phosphate buffer (10 : 90, v/v) pH 4.05, flow rate 0.6 mL min–1. UV detection was at 210 and 230 nm. The validation parameters showed good results: linearity (r >0.999) for all compounds, detection limits in the range 0.3–0.8 μg mL–1, repeatability (RSD) of peak heights between runs in the range 1.10–4.30% at three concentration levels and intra‐day repeatability of the retention times in the range 0.28–0.43%. The analysis time was
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200301644