Reduction of allergic airway eosinophilia by dietary raffinose in Brown Norway rats

Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-...

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Bibliographic Details
Published in:British journal of nutrition 2004-08, Vol.92 (2), p.247-255
Main Authors: Watanabe, Hiroshi, Sonoyama, Kei, Watanabe, Jun, Yamaguchi, Natsu, Kikuchi, Hiroto, Nagura, Taizo, Aritsuka, Tsutomu, Fukumoto, Kyoko, Kasai, Takanori
Format: Article
Language:English
Subjects:
Allergens
Allergens - immunology
Allergies
Allergy
Animals
Bacteria
Biological and medical sciences
Broncho-alveolar lavage fluid
Bronchoalveolar Lavage Fluid - immunology
Cell Count
Cytokines
Dermatitis
Diet
Dietary Supplements
Disease Models, Animal
Eczema
Eosinophilia
Eosinophilia - diet therapy
Eosinophilia - immunology
Feeding. Feeding behavior
Fundamental and applied biological sciences. Psychology
Human subjects
Immunization
Immunoglobulin E - analysis
Immunology
Interleukin-4 - analysis
Interleukin-5 - analysis
Lung - immunology
Lung - pathology
Macrophages, Alveolar - immunology
Male
Nutrition research
Oral administration
Ovalbumin
Prebiotics
Raffinose
Raffinose - administration & dosage
Raffinose - blood
Raffinose - immunology
Rats
Rats, Inbred BN
Respiratory Hypersensitivity - diet therapy
Respiratory Hypersensitivity - immunology
Reverse Transcriptase Polymerase Chain Reaction - methods
RNA, Messenger - analysis
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Oral administration of raffinose, a naturally occurring indigestible oligosaccharide, has reportedly ameliorated atopic dermatitis in human subjects although the mechanism is unknown. The present study investigated the effect of dietary raffinose on allergen-induced airway eosinophilia in ovalbumin-sensitised Brown Norway rats as an atopic disease model. Brown Norway rats were immunised by subcutaneous injection with ovalbumin on day 0 and fed either a control diet or the diet supplemented with raffinose (50 g/kg diet). The rats were exposed to aerosolised ovalbumin on day 20, and broncho-alveolar lavage fluid was obtained on the next day. The number of eosinophils in the fluid was significantly lower in the rats fed the raffinose diet than in those fed the control diet. Dietary raffinose significantly reduced IL-4 and IL-5 mRNA levels in lung tissue and tended to lower ovalbumin-specific Ig E levels. Suppression of eosinophilia by dietary raffinose was still observed in caecectomised and neomycin-administered rats, suggesting little contribution by the colonic bacteria to the effect of raffinose. Intraperitoneal administration of raffinose also suppressed eosinophilia. Significant concentrations of raffinose were detected in portal venous and abdominal arterial plasma after the intragastric administration of raffinose. Overall, the findings suggest that dietary raffinose ameliorates allergic airway eosinophilia at least partly via post-absorptive mechanisms in Brown Norway rats.
ISSN:0007-1145
1475-2662
DOI:10.1079/BJN20041179