Evaluation of tests for antibodies against bovine herpesvirus 1 performed in national reference laboratories in Europe

Sets of serum and milk samples were collected from various countries and prepared, lyophilised and distributed by 1 laboratory to 12 reference laboratories in Europe. The serum sets contained the three European bovine herpesvirus 1 (BHV1) reference serum samples (EU1, EU2 and EU3), serum samples fro...

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Bibliographic Details
Published in:Veterinary microbiology 2004-09, Vol.102 (3), p.169-181
Main Authors: Kramps, Johannes A., Banks, Malcolm, Beer, Martin, Kerkhofs, Pierre, Perrin, Myriam, Wellenberg, Gerard J., Oirschot, Jan T. Van
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - analysis
Antibodies, Viral - blood
Antibodies, Viral - isolation & purification
antibody detection
Biological and medical sciences
blood serum
Bovine herpesvirus 1
Cattle
ELISA
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - standards
Enzyme-Linked Immunosorbent Assay - veterinary
Europe
Fundamental and applied biological sciences. Psychology
Herpesvirus 1, Bovine - immunology
infectious bovine rhinotracheitis
Infectious Bovine Rhinotracheitis - diagnosis
laboratories
Laboratories - standards
Microbiology
milk
Milk - immunology
Milk - virology
Miscellaneous
neutralization tests
Neutralization Tests - methods
Neutralization Tests - standards
Neutralization Tests - veterinary
Quality
Quality Control
Reference Values
Ring test
ring tests
Sensitivity and Specificity
Vaccination - veterinary
vaginitis
vertebrate viruses
Viral Proteins - immunology
Viral Vaccines - immunology
Virology
Virus neutralisation test
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Summary:Sets of serum and milk samples were collected from various countries and prepared, lyophilised and distributed by 1 laboratory to 12 reference laboratories in Europe. The serum sets contained the three European bovine herpesvirus 1 (BHV1) reference serum samples (EU1, EU2 and EU3), serum samples from naturally and experimentally BHV1-infected cattle, from vaccinated, and vaccinated-challenged cattle, from uninfected cattle, and a series of serum dilutions. In addition, sets of milk samples were distributed. The samples were tested for antibodies against BHV1 in virus neutralisation tests, in gB-specific ELISAs, in indirect ELISAs and in gE-specific ELISAs. It was found that the virus neutralisation test and the gB-specific ELISAs were most sensitive for the detection of antibodies in serum, whereas for assaying milk samples the indirect ELISAs were the tests of choice. The results show that the quality of most laboratories appeared to be adequate, but that one laboratory performed considerably below an acceptable level of quality. Four samples from the panel have been proposed that might be selected as reference sera in addition to the three European reference samples.
ISSN:0378-1135
1873-2542
DOI:10.1016/j.vetmic.2004.07.003