Leading-edge myofibroblasts in human colon cancer express plasminogen activator inhibitor-1

Plasminogen activator inhibitor-1 (PAI-1) is up-regulated strongly in various cancer tissues, including colon cancer tissue. Highly specific rabbit polyclonal antibodies against PAI-1 were used for immunohistochemical localization of PAI-1 in 12 invasive colorectal adenocarcinomas. PAI-1 immunoreact...

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Bibliographic Details
Published in:American journal of clinical pathology 2004-08, Vol.122 (2), p.256-265
Main Authors: ILLEMANN, Martin, HANSEN, Ulla, NIELSEN, Hans Jørgen, ANDREASEN, Peter A, HØYER-HANSEN, Gunilla, LUND, Leif R, DANØ, Keld, NIELSEN, Boye Schnack
Format: Article
Language:English
Subjects:
Actins - metabolism
Adenocarcinoma - blood supply
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Antigens, CD34 - metabolism
Biological and medical sciences
Biomarkers, Tumor - analysis
Colonic Neoplasms - blood supply
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Endothelial Cells - metabolism
Fibroblasts - metabolism
Fibroblasts - pathology
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Immunohistochemistry
In Situ Hybridization
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Muscle Cells - metabolism
Muscle Cells - pathology
Muscle, Smooth - metabolism
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Plasminogen Activator Inhibitor 1 - biosynthesis
Plasminogen Activator Inhibitor 1 - immunology
RNA, Messenger - analysis
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
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Description
Summary:Plasminogen activator inhibitor-1 (PAI-1) is up-regulated strongly in various cancer tissues, including colon cancer tissue. Highly specific rabbit polyclonal antibodies against PAI-1 were used for immunohistochemical localization of PAI-1 in 12 invasive colorectal adenocarcinomas. PAI-1 immunoreactivity was observed in endothelial cells of some vessels located in the submucosa and in several fibroblast-like cells located at the invasive front. No PAI-1 immunoreactivity was seen in cancer cells in any of the 12 cases. Double immunofluorescence using the PAI-1 antibodies together with antibodies against alpha-smooth muscle actin for myofibroblast/smooth muscle cells and CD34 for endothelial cells showed that more than 80% of the PAI-1-positive fibroblast-like cells in all 12 cases were myofibroblasts. In 4 of 12 cases, a few of the PAI-1-positive fibroblast-like cells in the invasive front were CD34+. We conclude that the majority of PAI-1-positive fibroblast-like cells located at the leading edge of the invasive colon cancers are myofibroblasts.
ISSN:0002-9173
1943-7722
DOI:10.1309/F32XWQ20T568H8VP