Metabolic Profiling of Human Colorectal Cancer Using High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance (HR-MAS NMR) Spectroscopy and Gas Chromatography Mass Spectrometry (GC/MS)

Current clinical strategy for staging and prognostication of colorectal cancer (CRC) relies mainly upon the TNM or Duke system. This clinicopathological stage is a crude prognostic guide because it reflects in part the delay in diagnosis in the case of an advanced cancer and gives little insight int...

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Bibliographic Details
Published in:Journal of proteome research 2009-01, Vol.8 (1), p.352-361
Main Authors: Chan, Eric Chun Yong, Koh, Poh Koon, Mal, Mainak, Cheah, Peh Yean, Eu, Kong Weng, Backshall, Alexandra, Cavill, Rachel, Nicholson, Jeremy K, Keun, Hector C
Format: Article
Language:English
Subjects:
Biomarkers - analysis
Biomarkers - metabolism
Cell Line, Tumor
Colon - pathology
Colorectal Neoplasms - metabolism
Gas Chromatography-Mass Spectrometry - methods
Humans
Least-Squares Analysis
Magnetic Resonance Spectroscopy - methods
Metabolome
Metabolomics - methods
Prognosis
Rectum - pathology
Reproducibility of Results
ROC Curve
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Summary:Current clinical strategy for staging and prognostication of colorectal cancer (CRC) relies mainly upon the TNM or Duke system. This clinicopathological stage is a crude prognostic guide because it reflects in part the delay in diagnosis in the case of an advanced cancer and gives little insight into the biological characteristics of the tumor. We hypothesized that global metabolic profiling (metabonomics/metabolomics) of colon mucosae would define metabolic signatures that not only discriminate malignant from normal mucosae, but also could distinguish the anatomical and clinicopathological characteristics of CRC. We applied both high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) and gas chromatography mass spectrometry (GC/MS) to analyze metabolites in biopsied colorectal tumors and their matched normal mucosae obtained from 31 CRC patients. Orthogonal partial least-squares discriminant analysis (OPLS-DA) models generated from metabolic profiles obtained by both analytical approaches could robustly discriminate normal from malignant samples (Q 2 > 0.50, Receiver Operator Characteristic (ROC) AUC >0.95, using 7-fold cross validation). A total of 31 marker metabolites were identified using the two analytical platforms. The majority of these metabolites were associated with expected metabolic perturbations in CRC including elevated tissue hypoxia, glycolysis, nucleotide biosynthesis, lipid metabolism, inflammation and steroid metabolism. OPLS-DA models showed that the metabolite profiles obtained via HR-MAS NMR could further differentiate colon from rectal cancers (Q 2> 0.60, ROC AUC = 1.00, using 7-fold cross validation). These data suggest that metabolic profiling of CRC mucosae could provide new phenotypic biomarkers for CRC management.
ISSN:1535-3893
1535-3907
DOI:10.1021/pr8006232