MM3-ELISA evaluation of coproantigen release and serum antibody production in sheep experimentally infected with Fasciola hepatica and F. gigantica

During an experimental infection of sheep with Fasciola hepatica or F. gigantica, MM3-SERO and MM3-COPRO ELISA tests were applied to compare the kinetics of antibody production and coproantigen release between the 2nd and 32nd week post-infection (wpi). The Kato-Katz technique was used to measure th...

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Bibliographic Details
Published in:Veterinary parasitology 2009-01, Vol.159 (1), p.77-81
Main Authors: Valero, M. Adela, Ubeira, Florencio M., Khoubbane, Messaoud, Artigas, Patricio, Muiño, Laura, Mezo, Mercedes, Pérez-Crespo, Ignacio, Periago, M. Victoria, Mas-Coma, Santiago
Format: Article
Language:English
Subjects:
absorbance
Adults
Animals
Antibodies, Helminth - blood
Antibodies, Helminth - immunology
Antibodies, Monoclonal - immunology
antibody formation
Antibody Formation - immunology
Antigens, Helminth - analysis
Antigens, Helminth - immunology
coproantigens
disease diagnosis
Eggs
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzyme-Linked Immunosorbent Assay - veterinary
Experimental infection
Fasciola - immunology
Fasciola gigantica
Fasciola hepatica
Fasciola hepatica - immunology
Fasciola hepatica - isolation & purification
fascioliasis
Fascioliasis - blood
Fascioliasis - immunology
Fascioliasis - veterinary
fecal egg count
Feces - parasitology
immunoglobulin G
Immunoglobulin G - immunology
Kato-Katz technique
Kinetics
Linear Models
MM3-COPRO enzyme-linked immunosorbent assay
MM3-SERO and MM3-COPRO ELISA tests
Parasite Egg Count - veterinary
Random Allocation
Sheep
Sheep - immunology
sheep diseases
Sheep Diseases - diagnosis
Sheep Diseases - immunology
Sheep Diseases - parasitology
Statistics, Nonparametric
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Summary:During an experimental infection of sheep with Fasciola hepatica or F. gigantica, MM3-SERO and MM3-COPRO ELISA tests were applied to compare the kinetics of antibody production and coproantigen release between the 2nd and 32nd week post-infection (wpi). The Kato-Katz technique was used to measure the kinetics of egg shedding by both Fasciola species (eggs per gram of feces, epg). The kinetics of IgG antibodies for all sheep infected with F. hepatica and F. gigantica followed a similar pattern. Optical density (OD) increased rapidly between the 4th until the 12th wpi, when the highest values were reached and then decreased slowly until the 32nd wpi. Coproantigen levels increased above the cut-off value between 6 and 9 wpi in the F. hepatica group, and between 9 and 11 wpi in the F. gigantica group. The comparison between coproantigen levels and epg indicated that F. hepatica-infected sheep had detectable amounts of coproantigens 4–7 weeks before patency (egg shedding), while F. gigantica-infected sheep had detectable amounts of coproantigens 3–6 weeks before patency. When comparing the kinetics of coproantigen release vs the kinetics of epg, a similar pattern emerged, but with a two-week time-lag in epg, for both F. hepatica and F. gigantica infections. The amount of coproantigen release by each adult was not burden dependent for F. hepatica infection (burden of 33–66 adults), while it was for F. gigantica infection (burden of 17–69 adults). The results demonstrate the usefulness of the MM3-SERO and MM3-COPRO ELISAs as tools for the diagnosis of early as well as long-term fascioliasis infections, and suggest that they can potentially be applied to human fascioliasis even in countries where F. hepatica and F. gigantica co-exist. These tests can be employed not only in the diagnosis, but also in studies on epidemiology as well as pathogenesis and treatment in animals and humans since they allow post-treatment infection monitoring.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2008.10.014