Estrogen replacement treatment in diabetic ovariectomized female rats potentiates postischemic leukocyte adhesion in cerebral venules

Chronic 17beta-estradiol (E2) replacement therapy in ovariectomized (OVX) female rats reduces leukocyte adhesion and brain damage after transient forebrain ischemia. Recently, we found that E2 treatment in diabetic OVX females was associated with enhanced postischemic neuropathology. We tested the h...

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Bibliographic Details
Published in:Stroke (1970) 2004-08, Vol.35 (8), p.1974-1978
Main Authors: XU, Hao-Liang, BAUGHMAN, Verna L, PELLIGRINO, Dale A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain Ischemia - immunology
Brain Ischemia - physiopathology
Cell Adhesion
Cerebrovascular Circulation
Diabetes Mellitus, Experimental
Estradiol - pharmacology
Estrogen Replacement Therapy
Female
Hyperglycemia - immunology
Hyperglycemia - physiopathology
Leukocytes - physiology
Medical sciences
Neurology
Ovariectomy
Rats
Rats, Sprague-Dawley
Vascular diseases and vascular malformations of the nervous system
Venules
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Summary:Chronic 17beta-estradiol (E2) replacement therapy in ovariectomized (OVX) female rats reduces leukocyte adhesion and brain damage after transient forebrain ischemia. Recently, we found that E2 treatment in diabetic OVX females was associated with enhanced postischemic neuropathology. We tested the hypothesis that in chronically hyperglycemic diabetic OVX females, chronic E2 replacement potentiates post-transient forebrain ischemia leukocyte adhesion. Pial venules were observed through closed cranial windows. Adherence of rhodamine 6G-tagged leukocytes was monitored before and 10 hours after transient forebrain ischemia (20 minutes right common carotid artery occlusion plus hemorrhagic hypotension) in intact, untreated OVX and E2-treated OVX females rendered diabetic via streptozotocin. Leukocyte adhesion was quantitated as the percentage venular area occupied by adherent leukocytes. At 2 hours after transient forebrain ischemia, a similar low level of leukocyte adhesion was seen in the 3 groups (
ISSN:0039-2499
1524-4628
DOI:10.1161/01.STR.0000135016.24349.9F