Higher levels of salivary MUC5B and MUC7 in individuals with gastric diseases who harbor Helicobacter pylori

Abstract Objective The aim of the present study was to assess the salivary levels of MUC5B and MUC7 in individuals with dyspeptic disease and Helicobacter pylori ( H. pylori ) in the stomach , compared to individuals without dyspeptic disease. Methods 30 individuals with dyspeptic disease, who under...

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Bibliographic Details
Published in:Archives of oral biology 2009-01, Vol.54 (1), p.86-90
Main Authors: Silva, Denise G, Stevens, Roy H, Macedo, Jacyara M.B, Hirata, Raphael, Pinto, Amanda C, Alves, Luciano M, Veerman, Enno C.I, Tinoco, Eduardo M.B
Format: Article
Language:English
Subjects:
Advanced Basic Science
Biomarkers - analysis
Brazil
Case-Control Studies
Colony Count, Microbial
Dentistry
Dyspepsia - metabolism
Dyspepsia - microbiology
ELISA
Enzyme-Linked Immunosorbent Assay
Female
Gastric disease
Helicobacter Infections - metabolism
Helicobacter pylori
Helicobacter pylori - isolation & purification
Humans
Male
Middle Aged
Mucin-5B - analysis
Mucins
Mucins - analysis
PCR
Saliva
Saliva - chemistry
Saliva - microbiology
Salivary Proteins and Peptides - analysis
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Summary:Abstract Objective The aim of the present study was to assess the salivary levels of MUC5B and MUC7 in individuals with dyspeptic disease and Helicobacter pylori ( H. pylori ) in the stomach , compared to individuals without dyspeptic disease. Methods 30 individuals with dyspeptic disease, who underwent endoscopy for upper gastrointestinal complaints at Hospital Pedro Ernesto-RJ, Brasil and tested positive for H. pylori , and 23 controls with no dyspeptic disease, with mean age 53.5 ± 4.4 years, were included in the study. Saliva samples and 3 antral biopsy were taken for PCR analysis and histologic examination. In addition, saliva samples were tested by ELISA with F2 monoclonal antibody and EU7A antibody against MUC7, to determine MUC5B and MUC7 levels, prior to endoscopic examination. The expression pattern of the proteins was quantified by comparison to a pooled saliva sample of 19 healthy volunteers. Results MUC5B and MUC7 salivary levels were higher in the individuals with dyspeptic disease than in controls ( p < 0.0001). 33.3% (9/30) of the dyspeptic individuals and 0% of the controls had H. pylori in the oral cavity. Conclusions Individuals with gastric diseases, with H. pylori in the stomach, showed higher levels of salivary H. pylori receptors–MUC5B and MUC7–than individuals without gastric diseases. These results suggest that higher levels of specific salivary mucins could be useful as risk indicators for infection by H. pylori.
ISSN:0003-9969
1879-1506
DOI:10.1016/j.archoralbio.2008.08.003