The in vivo stability of electrospun polycaprolactone-collagen scaffolds in vascular reconstruction

Abstract To avoid complications of prosthetic vascular grafts, engineered vascular constructs have been investigated as an alternative for vascular reconstruction. The scaffolds for vascular tissue engineering remain a cornerstone of these efforts and yet many currently available options are limited...

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Bibliographic Details
Published in:Biomaterials 2009-02, Vol.30 (4), p.583-588
Main Authors: Tillman, Bryan W, Yazdani, Saami K, Lee, Sang Jin, Geary, Randolph L, Atala, Anthony, Yoo, James J
Format: Article
Language:English
Subjects:
Advanced Basic Science
Animals
Biomechanical Phenomena
Blood Vessel Prosthesis
Blood Vessels - metabolism
Cell Adhesion
Collagen
Collagen - metabolism
Dentistry
Electrospinning
Endothelial Cells - cytology
Endothelial Cells - ultrastructure
Materials Testing
Myocytes, Smooth Muscle - cytology
Platelet Adhesiveness
Polycaprolactone
Polyesters - metabolism
Reconstructive Surgical Procedures
Sheep
Tissue engineering
Tissue Scaffolds
Vascular graft
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Summary:Abstract To avoid complications of prosthetic vascular grafts, engineered vascular constructs have been investigated as an alternative for vascular reconstruction. The scaffolds for vascular tissue engineering remain a cornerstone of these efforts and yet many currently available options are limited by issues of inconsistency, poor adherence of vascular cells, or inadequate biomechanical properties. In this study, we investigated whether PCL/collagen scaffolds could support cell growth and withstand physiologic conditions while maintaining patency in a rabbit aortoiliac bypass model. Our results indicate that electrospun scaffolds support adherence and growth of vascular cells under physiologic conditions and that endothelialized grafts resisted adherence of platelets when exposed to blood. When implanted in vivo , these scaffolds were able to retain their structural integrity over 1 month of implantation as demonstrated by serial ultrasonography. Further, at retrieval, these scaffolds continued to maintain biomechanical strength that was comparable to native artery. This study suggests that electrospun scaffolds combined with vascular cells may become an alternative to prosthetic vascular grafts for vascular reconstruction.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2008.10.006